Abstract：
This aim of this study was to assess anti-parasitic properties of deep eutectic solvents against eye pathogen, Acanthamoeba, often associated with the use of contact lens.
Assays were performed to investigate the effects of various Methyltrioctylammonium chloride-based deep eutectic solvents on Acanthamoeba castellanii, comprising amoebicidal assays, encystment assays, excystment assays, cytotoxicity assays by measuring lactate dehydrogenase release from human cells, and cytopathogenicity assays to determine parasite-mediated host cell death.
In a 2 h incubation period, DES-B, DES-C, DES-D, and DES-E exhibited up to 85 % amoebicidal activity at micromolar doses, which was enhanced further following 24 h incubation. When tested in encystment assays, selected deep eutectic solvents abolished cyst formation and were able to block excystment of A. castellanii. All solvents exhibited minimal human cell cytotoxicity except DES-D. Finally, all tested deep eutectic solvents inhibited amoeba-mediated cytopathogenicity, except DES-B.
Deep eutectic solvents show potent antiamoebic effects. These findings are promising and could lead to the development of novel contact lens disinfectants, as well as opening several avenues to explore the molecular mechanisms, various doses and incubation periods, and use of different bases against Acanthamoeba castellanii.
Assays were performed to investigate the effects of various Methyltrioctylammonium chloride-based deep eutectic solvents on Acanthamoeba castellanii, comprising amoebicidal assays, encystment assays, excystment assays, cytotoxicity assays by measuring lactate dehydrogenase release from human cells, and cytopathogenicity assays to determine parasite-mediated host cell death.
In a 2 h incubation period, DES-B, DES-C, DES-D, and DES-E exhibited up to 85 % amoebicidal activity at micromolar doses, which was enhanced further following 24 h incubation. When tested in encystment assays, selected deep eutectic solvents abolished cyst formation and were able to block excystment of A. castellanii. All solvents exhibited minimal human cell cytotoxicity except DES-D. Finally, all tested deep eutectic solvents inhibited amoeba-mediated cytopathogenicity, except DES-B.
Deep eutectic solvents show potent antiamoebic effects. These findings are promising and could lead to the development of novel contact lens disinfectants, as well as opening several avenues to explore the molecular mechanisms, various doses and incubation periods, and use of different bases against Acanthamoeba castellanii.
摘要：
目的：本研究的目的是评估低共熔溶剂对眼部病原体的抗寄生虫特性，棘阿米巴,经常与隐形眼镜的使用有关。
方法：进行了试验以研究各种基于甲基三辛基氯化铵的低共熔溶剂对棘阿米巴的影响，包括杀变形虫试验，包膜测定，包装测定，通过测量人体细胞乳酸脱氢酶释放的细胞毒性试验，和细胞致病性测定以确定寄生虫介导的宿主细胞死亡。
结果：在2小时的潜伏期中，DES-B,DES-C,DES-D,DES-E在微摩尔剂量下表现出高达85％的杀变形虫活性，孵育24小时后进一步增强。当在包封测定中测试时，选择的低共熔溶剂消除了囊肿的形成，并能够阻止A.castellanii的分泌。除DES-D外，所有溶剂均表现出最小的人细胞毒性。最后,所有测试的低共熔溶剂均抑制变形虫介导的细胞致病性，除了DES-B
结论：深共晶溶剂显示出有效的抗阿米巴作用。这些发现是有希望的，并可能导致新型隐形眼镜消毒剂的发展，以及打开几个途径来探索分子机制，各种剂量和潜伏期，并使用不同的基地对抗棘阿米巴。
方法：进行了试验以研究各种基于甲基三辛基氯化铵的低共熔溶剂对棘阿米巴的影响，包括杀变形虫试验，包膜测定，包装测定，通过测量人体细胞乳酸脱氢酶释放的细胞毒性试验，和细胞致病性测定以确定寄生虫介导的宿主细胞死亡。
结果：在2小时的潜伏期中，DES-B,DES-C,DES-D,DES-E在微摩尔剂量下表现出高达85％的杀变形虫活性，孵育24小时后进一步增强。当在包封测定中测试时，选择的低共熔溶剂消除了囊肿的形成，并能够阻止A.castellanii的分泌。除DES-D外，所有溶剂均表现出最小的人细胞毒性。最后,所有测试的低共熔溶剂均抑制变形虫介导的细胞致病性，除了DES-B
结论：深共晶溶剂显示出有效的抗阿米巴作用。这些发现是有希望的，并可能导致新型隐形眼镜消毒剂的发展，以及打开几个途径来探索分子机制，各种剂量和潜伏期，并使用不同的基地对抗棘阿米巴。
