PDF(Pubmed)
Abstract：
CTP synthase (CTPS) can form filamentous structures termed cytoophidia in cells in all three domains of life. In order to study the mesoscale structure of cytoophidia, we perform fluorescence recovery after photobleaching (FRAP) and stimulated emission depletion (STED) microscopy in human cells. By using an EGFP dimeric tag as a tool to explore the physical properties of cytoophidia, we find that cytoophidia are dynamic and reticular. The reticular structure of CTPS cytoophidia may provide space for other components, such as IMPDH. In addition, we observe CTPS granules with tentacles.
摘要：
CTP合酶（CTPS）可以在生命的所有三个域中的细胞中形成丝状结构，称为胞嘧啶。为了研究胞嘧啶的中尺度结构，我们在人类细胞中进行光漂白（FRAP）和受激发射损耗（STED）显微镜检查后进行荧光恢复。通过使用EGFP二聚体标签作为工具来探索胞质的物理性质，我们发现胞质是动态的和网状的。CTPS胞质的网状结构可能为其他成分提供空间，比如IMPDH。此外,我们观察到有触手的CTPS颗粒。
