Abstract：
Anaplasma genus infects the blood cells of humans and animals by biting, causing zoonotic anaplasmosis. However, limited data are available on carrier animals for Anaplasma spp. antibodies in the Qinghai−Tibetan Plateau Area. Therefore, a serological indirect ELISA diagnostic method based on the major surface protein 5 (MSP5), derived from Anaplasma phagocytophilum, was developed in this study to analyze both IgG and IgM antibodies of Anaplasma spp. in a total of 3952 animals from the Qinghai−Tibetan Plateau, including yaks (Bos grunniens), cows (Bos taurus), cattle (Bos taurus domesticus), Tibetan sheep (Ovis aries), horses (Equus ferus caballus), pigs (Sus domesticus), chickens (Gallus gallus domesticus), donkeys (Equus asinus), stray dogs (Canis sp.), and stray cats (Felis sp.). The results showed that recombinant MSP5 protein was expressed and was successfully used to establish the indirect ELISA methods. The overall positivity for Anaplasma IgG and IgM antibodies was 14.6% (578/3952) and 7.9% (312/3952), respectively, and a total of 123 animals (3.1%) were both IgG- and IgM-positive. Moreover, the most prevalent Anaplasma IgG positivity was exhibited by donkeys (82.5%), followed by stray dogs, Tibetan sheep, pigs, chickens, horses, yaks, cows, cattle, and stray cats. The analysis for IgM antibody positivity revealed that IgM positivity was the most prevalent in the stray dogs (30.1%), followed by horses, yaks, Tibetan sheep, cows, stray cats, and cattle. Moreover, the results revealed significant differences (p < 0.05) at different altitudes in Anaplasma-specific IgG in the yaks, Tibetan sheep, and horses, and in IgM in the yaks and Tibetan sheep. In conclusion, this study is the first to demonstrate that yaks, cows, cattle, Tibetan sheep, horses, donkeys, stray dogs, stray cats, pigs, and chickens living in the Qinghai−Tibet Plateau are carrier animals for Anaplasma spp. IgG or IgM antibodies. The current findings provide valuable current data on the seroepidemiology of anaplasmosis in China and for plateau areas of the world.
摘要：
无性体属通过叮咬感染人类和动物的血细胞，导致人畜共患无形体病.然而,关于无性体属的载体动物的数据有限。青藏高原地区的抗体。因此,基于主要表面蛋白5（MSP5）的血清学间接ELISA诊断方法，来自吞噬细胞无性体，在这项研究中开发了用于分析无性体属的IgG和IgM抗体。在来自青藏高原的3952只动物中，包括牦牛（Bosgrunniens），牛（Bostaurus），牛（Bostaurusdomesticus），藏羊（Ovisaries），马（马），猪（Susdomesticus），鸡（家鸡），驴(马),流浪狗(犬类),和流浪猫（Felissp。).结果表明，表达了重组MSP5蛋白，并成功建立了间接ELISA方法。无性体IgG和IgM抗体的总体阳性率为14.6%(578/3952)和7.9%(312/3952),分别,共有123只动物(3.1%)为IgG和IgM阳性。此外，最普遍的抗体IgG阳性表现为驴（82.5%），接下来是流浪狗，西藏羊,猪,鸡,马,牦牛,奶牛,牛,和流浪猫。IgM抗体阳性分析显示，IgM阳性在流浪狗中最为普遍（30.1%），跟着马,牦牛,西藏羊,奶牛,流浪猫,和牛。此外，结果显示不同海拔高度牦牛体内无性体特异性IgG存在显著差异(p<0.05),西藏羊,和马,牦牛和藏羊的IgM。总之,这项研究首次证明牦牛，奶牛,牛,西藏羊,马,驴,流浪狗,流浪猫,猪,生活在青藏高原的鸡是anap理虫的载体动物。IgG或IgM抗体。目前的发现为中国和世界高原地区的无形体病血清流行病学提供了宝贵的最新数据。
