Abstract：
Owing to the greater absorption affinity for lipo-rich tissue than water, the 1210 nm laser is a promising candidate for transcutaneous lipolysis in the near-infrared band. However, fat reduction is limited because laser therapy may yield thermal injury of normal tissue. A new protocol to incorporate multipulsed cryogen spray cooling is beneficial to improve the lipolysis effect, and the parameters of laser and cooling can be optimized via skin histopathological analysis.
A murine in vivo model of inguinal tissue of SD rats was established to test the effectivity of transcutaneous lipolysis protocol by R134a multipulsed spray cooling assisted 1210 nm laser irradiation. Tissue response of lipolysis with/without cooling 10 days post the treatment was evaluated by histopathological analysis of skin samples stained with hematoxylin-eosin (HE), through which safe and effective parameters for lipolysis were determined.
From histopathological analysis of the inguinal tissue of SD rats irradiated by the 1210 nm laser alone, the optimal durations are respectively 7 and 3 s (seconds) for low-dosage (6 W) and high-dosage (9 W) therapy, with pronounced lipolysis effect and minimum injury of skin tissue. The multipulsed spray cooling by R134a with a pulse duration of 10 ms (milliseconds), a pulse delay of 2000 ms, and a pulse number of 5 can be introduced to assist the 1210 nm laser therapy with a power of 9 W and a duration of 7 s to achieve desirable fat liquefaction while keeping the complete structure of skin tissue as well as esthetic-related beneficial effects of hair removal and skin rejuvenation.
Excellent lipolysis effect can be achieved via R134a multipulsed spray cooling assisted high-dosage 1210 nm laser irradiation with reasonably matched laser and cooling parameters. The protocol is as follows: Start MP-CSC for one cycle, and then fire the laser with specific power and duration, while keeping MP-CSC accordingly. This new protocol may promote the safe and effective clinical implement of transcutaneous laser lipolysis in body contouring.
A murine in vivo model of inguinal tissue of SD rats was established to test the effectivity of transcutaneous lipolysis protocol by R134a multipulsed spray cooling assisted 1210 nm laser irradiation. Tissue response of lipolysis with/without cooling 10 days post the treatment was evaluated by histopathological analysis of skin samples stained with hematoxylin-eosin (HE), through which safe and effective parameters for lipolysis were determined.
From histopathological analysis of the inguinal tissue of SD rats irradiated by the 1210 nm laser alone, the optimal durations are respectively 7 and 3 s (seconds) for low-dosage (6 W) and high-dosage (9 W) therapy, with pronounced lipolysis effect and minimum injury of skin tissue. The multipulsed spray cooling by R134a with a pulse duration of 10 ms (milliseconds), a pulse delay of 2000 ms, and a pulse number of 5 can be introduced to assist the 1210 nm laser therapy with a power of 9 W and a duration of 7 s to achieve desirable fat liquefaction while keeping the complete structure of skin tissue as well as esthetic-related beneficial effects of hair removal and skin rejuvenation.
Excellent lipolysis effect can be achieved via R134a multipulsed spray cooling assisted high-dosage 1210 nm laser irradiation with reasonably matched laser and cooling parameters. The protocol is as follows: Start MP-CSC for one cycle, and then fire the laser with specific power and duration, while keeping MP-CSC accordingly. This new protocol may promote the safe and effective clinical implement of transcutaneous laser lipolysis in body contouring.
摘要：
目的：由于对富含脂肪的组织比水具有更大的吸收亲和力，1210nm激光是近红外波段经皮脂肪分解的有希望的候选者。然而,由于激光治疗可能会对正常组织产生热损伤，因此脂肪减少是有限的。一种新的协议，纳入多脉冲制冷剂喷雾冷却是有益的，以提高脂解效果，通过皮肤组织病理学分析可以优化激光和冷却的参数。
方法：建立SD大鼠腹股沟组织的小鼠体内模型，以测试R134a多脉冲喷雾冷却辅助1210nm激光照射经皮脂肪分解方案的有效性。通过用苏木精-伊红（HE）染色的皮肤样品的组织病理学分析评估治疗后10天有/没有冷却的脂解的组织反应，通过该方法确定了安全有效的脂解参数。
结果：通过对1210nm激光照射的SD大鼠腹股沟组织的组织病理学分析，低剂量（6W）和高剂量（9W）治疗的最佳持续时间分别为7和3s（秒），具有明显的脂解作用和最小的皮肤组织损伤。R134a的多脉冲喷雾冷却，脉冲持续时间为10ms(毫秒)，2000毫秒的脉冲延迟，并且可以引入5的脉冲数以辅助功率为9W和持续时间为7s的1210nm激光治疗,以实现期望的脂肪液化,同时保持皮肤组织的完整结构以及毛发去除和皮肤恢复的美学相关有益效果。
结论：通过合理匹配的激光和冷却参数，通过R134a多脉冲喷雾冷却辅助高剂量1210nm激光照射可以实现出色的脂解效果。协议如下：启动MP-CSC一个周期，然后以特定的功率和持续时间发射激光，同时相应地保持MP-CSC。这种新方案可能会促进经皮激光脂解在身体轮廓中的安全有效的临床实施。
方法：建立SD大鼠腹股沟组织的小鼠体内模型，以测试R134a多脉冲喷雾冷却辅助1210nm激光照射经皮脂肪分解方案的有效性。通过用苏木精-伊红（HE）染色的皮肤样品的组织病理学分析评估治疗后10天有/没有冷却的脂解的组织反应，通过该方法确定了安全有效的脂解参数。
结果：通过对1210nm激光照射的SD大鼠腹股沟组织的组织病理学分析，低剂量（6W）和高剂量（9W）治疗的最佳持续时间分别为7和3s（秒），具有明显的脂解作用和最小的皮肤组织损伤。R134a的多脉冲喷雾冷却，脉冲持续时间为10ms(毫秒)，2000毫秒的脉冲延迟，并且可以引入5的脉冲数以辅助功率为9W和持续时间为7s的1210nm激光治疗,以实现期望的脂肪液化,同时保持皮肤组织的完整结构以及毛发去除和皮肤恢复的美学相关有益效果。
结论：通过合理匹配的激光和冷却参数，通过R134a多脉冲喷雾冷却辅助高剂量1210nm激光照射可以实现出色的脂解效果。协议如下：启动MP-CSC一个周期，然后以特定的功率和持续时间发射激光，同时相应地保持MP-CSC。这种新方案可能会促进经皮激光脂解在身体轮廓中的安全有效的临床实施。
