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Abstract：
Background Chitosan is a biocompatible, biodegradable, and non-toxic natural polymer that can be fabricated by different methods for use in dental and biomedical fields. Electrospinning can produce polymeric nanofibrous scaffolds and membranes with desirable properties for use in tissue engineering. The objectives of this study were to investigate several morphological, physical, and biological characteristics of these nanofibrous scaffolds and evaluate their potential use in tissue engineering. Methodology Chitosan/polyvinyl alcohol nanofibrous scaffolds (CS/PVA NFS) in a ratio of 70/30 were fabricated by conventional electrospinning. The scaffolds were evaluated chemically by Fourier transformed infrared spectroscopy (FTIR) and morphologically by the atomic force microscope (AFM) and the field emission-scanning electron microscope (FE-SEM). These scaffolds were also evaluated mechanically by a tensile strength test and several investigations, including water contact angle, swelling ratio, and degradation ratio. Biological evaluations included protein adsorption, cell culture, and cell viability assay. Results The morphological evaluation revealed a homogenous, bead-free mat with an average fiber diameter of 172.7 ± 56.8 nm, an average pore size of 0.54 ± 0.17 µm, and porosity of 74.8% ± 3.3%; the scaffolds showed a tensile strength of 6.67 ± 0.7 Mpa. Scaffolds showed a desired hydrophilic property, as shown by the water contact angle test with a mean angle of 29.5°, while the swelling ratio was 229%, and degradability in phosphate buffer solution after 30 days was 26.9 ± 2.9%. In-vitro cell culture study with adipose tissue mesenchymal stem cells and cell viability and cytotoxicity tests by MTT assay demonstrated well-attached cells with increasing proliferation rate with no signs of cytotoxicity. Conclusions Assessment of the CS/PVA NFS revealed randomly oriented bead-free and porous mats. The scaffolds were stable at aqueous solutions following thermal treatment. They were hydrophilic, biodegradable, and biocompatible, as shown by the cell culture and MTT assay, which suggest that the fabricated scaffolds have the potential to be used in tissue engineering applications either as scaffolds, bio-grafts, or barrier membranes.
摘要：
背景壳聚糖是一种生物相容性,可生物降解，和无毒的天然聚合物，可以通过不同的方法制造，用于牙科和生物医学领域。静电纺丝可以产生具有用于组织工程的所需性质的聚合物纳米纤维支架和膜。这项研究的目的是调查几种形态学，物理，和这些纳米纤维支架的生物学特性，并评估其在组织工程中的潜在用途。方法通过常规静电纺丝制备比例为70/30的壳聚糖/聚乙烯醇纳米纤维支架（CS/PVANFS）。支架通过傅立叶变换红外光谱（FTIR）进行化学评估，并通过原子力显微镜（AFM）和场发射扫描电子显微镜（FE-SEM）进行形态学评估。这些支架还通过拉伸强度测试和一些调查进行了机械评估，包括水接触角，溶胀率，和降解率。生物学评估包括蛋白质吸附，细胞培养,和细胞活力测定。结果形态学评价显示，无珠垫，平均纤维直径为172.7±56.8nm，平均孔径为0.54±0.17µm，孔隙率为74.8%±3.3%；支架的拉伸强度为6.67±0.7Mpa。支架显示了所需的亲水性，如平均角度为29.5°的水接触角试验所示，而溶胀率为229%，30天后在磷酸盐缓冲溶液中的降解率为26.9±2.9％。用脂肪组织间充质干细胞进行的体外细胞培养研究以及通过MTT测定进行的细胞活力和细胞毒性测试表明，细胞附着良好，增殖率增加，没有细胞毒性迹象。结论CS/PVANFS的评估显示了随机取向的无珠和多孔垫。支架在热处理后在水溶液中是稳定的。它们是亲水的，可生物降解，和生物相容性，如细胞培养和MTT分析所示，这表明制造的支架有可能作为支架用于组织工程应用，生物移植物，或屏障膜。
