Abstract：
BACKGROUND: The OSNA technique is based on reverse transcription loop-mediated DNA amplification for the detection of cytokeratin 19 (CK19) messen-ger RNA (mRNA). The purpose of our paper, which represents the first study in the literature, is to test the accuracy of this method in the detection of lymph node metastases in patients undergoing robotic radical prostatectomy with lymph node dis-section.
METHODS: Our cohort consisted of patients that have undergone robotic radical prostatectomy with extended lymph node dissec-tion. Lymph nodes were evaluated with imprint technique and then with frozen section examination. The remaining tissue was evaluated by OSNA method. Lymph nodes were defined as \'neg-ative\' or \'positive\' according to mRNA copy number.
RESULTS: 7 patients and 25 lymph nodes were included in our cohort. Two patients were found negative with all pathology methods. In one patient the standard stains revealed a suspi-cious outcome but it was positive for micrometastasis with OSNA. In another patient the outcome was positive for standard stains and negative for OSNA. Finally, 2 patients were found positive for OSNA and negative for imprint methods.
CONCLUSIONS: One Step Nucleic Acid Amplification (OSNA) method using CK19 seems to fail in detection of lymph node metastases in prostate cancer patients undergoing radical prostatectomy and lymph node dissection.
METHODS: Our cohort consisted of patients that have undergone robotic radical prostatectomy with extended lymph node dissec-tion. Lymph nodes were evaluated with imprint technique and then with frozen section examination. The remaining tissue was evaluated by OSNA method. Lymph nodes were defined as \'neg-ative\' or \'positive\' according to mRNA copy number.
RESULTS: 7 patients and 25 lymph nodes were included in our cohort. Two patients were found negative with all pathology methods. In one patient the standard stains revealed a suspi-cious outcome but it was positive for micrometastasis with OSNA. In another patient the outcome was positive for standard stains and negative for OSNA. Finally, 2 patients were found positive for OSNA and negative for imprint methods.
CONCLUSIONS: One Step Nucleic Acid Amplification (OSNA) method using CK19 seems to fail in detection of lymph node metastases in prostate cancer patients undergoing radical prostatectomy and lymph node dissection.
摘要：
背景：OSNA技术基于逆转录环介导的DNA扩增，用于检测细胞角蛋白19（CK19）信使RNA（mRNA）。我们论文的目的,这代表了文献中fi的第一项研究，是为了测试这种方法在接受机器人根治性前列腺切除术并进行淋巴结切除的患者中淋巴结转移检测的准确性。
方法：我们的队列包括接受机器人根治性前列腺切除术并扩大淋巴结清扫的患者。用压印技术评估淋巴结，然后用冷冻切片检查。其余组织采用OSNA法进行评价。根据mRNA拷贝数，淋巴结被定义为“阴性”或“阳性”。
结果：我们的队列包括7例患者和25个淋巴结。2例患者所有病理检查均为阴性。在一名患者中，标准染色显示出可疑的结果，但对OSNA的微转移呈阳性。在另一位患者中，标准染色结果为阳性，OSNA结果为阴性。最后,发现2例患者OSNA阳性，印迹方法阴性。
结论：使用CK19的一步法核酸扩增（OSNA）方法似乎无法检测前列腺癌患者接受根治性前列腺切除术和淋巴结清扫术的淋巴结转移。
方法：我们的队列包括接受机器人根治性前列腺切除术并扩大淋巴结清扫的患者。用压印技术评估淋巴结，然后用冷冻切片检查。其余组织采用OSNA法进行评价。根据mRNA拷贝数，淋巴结被定义为“阴性”或“阳性”。
结果：我们的队列包括7例患者和25个淋巴结。2例患者所有病理检查均为阴性。在一名患者中，标准染色显示出可疑的结果，但对OSNA的微转移呈阳性。在另一位患者中，标准染色结果为阳性，OSNA结果为阴性。最后,发现2例患者OSNA阳性，印迹方法阴性。
结论：使用CK19的一步法核酸扩增（OSNA）方法似乎无法检测前列腺癌患者接受根治性前列腺切除术和淋巴结清扫术的淋巴结转移。
