Abstract：
DNA data storage technology may supersede conventional chip or magnetic data storage medium, providing long-term stability, high density, and sustainable storage. Due to its error-correcting capability, DNA data stored in living organisms exhibits high fidelity in information replication. Here we report the development of a Bacillus chassis integrated with an inducible artificially assembled bacterial chromosome to facilitate random data access. We generated three sets of data in the form of DNA sequences using a rudimentary coding system accessible by the regulatory promoter. Sporulated Bacillus harboring the genes were used for long-term storage, where viability assays of spores were subjected to harsh environmental stresses to evaluate the data storage stability. The data accuracy remained above 99% after high temperature and oxidative stress treatment, whereas UV irradiation treatment provided above 96% accuracy. The developed Bacillus chassis and artificial chromosome facilitate the long-term storage of larger datum volume by using other DNA digital encoding and decoding programs.
摘要：
DNA数据存储技术可以取代传统的芯片或磁性数据存储介质，提供长期稳定性，高密度,可持续存储。由于其纠错能力，存储在活生物体中的DNA数据在信息复制中表现出高保真度。在这里，我们报告了与可诱导的人工组装细菌染色体集成的芽孢杆菌底盘的开发，以促进随机数据访问。我们使用调控启动子可访问的基本编码系统以DNA序列的形式生成了三组数据。带有这些基因的孢子芽孢杆菌用于长期储存，其中孢子的活力测定经受恶劣的环境压力以评估数据存储稳定性。高温和氧化应激处理后，数据精度保持在99%以上，而紫外线照射处理提供了96%以上的准确度。开发的芽孢杆菌底盘和人工染色体通过使用其他DNA数字编码和解码程序来促进较大数据量的长期存储。
