{Reference Type}: Journal Article
{Title}: Engineered Spore-Forming Bacillus as a Microbial Vessel for Long-Term DNA Data Storage.
{Author}: Liu F;Li J;Zhang T;Chen J;Ho CL;
{Journal}: ACS Synth Biol
{Volume}: 11
{Issue}: 11
{Year}: 11 2022 18
{Factor}: 5.249
{DOI}: 10.1021/acssynbio.2c00291
{Abstract}: DNA data storage technology may supersede conventional chip or magnetic data storage medium, providing long-term stability, high density, and sustainable storage. Due to its error-correcting capability, DNA data stored in living organisms exhibits high fidelity in information replication. Here we report the development of a Bacillus chassis integrated with an inducible artificially assembled bacterial chromosome to facilitate random data access. We generated three sets of data in the form of DNA sequences using a rudimentary coding system accessible by the regulatory promoter. Sporulated Bacillus harboring the genes were used for long-term storage, where viability assays of spores were subjected to harsh environmental stresses to evaluate the data storage stability. The data accuracy remained above 99% after high temperature and oxidative stress treatment, whereas UV irradiation treatment provided above 96% accuracy. The developed Bacillus chassis and artificial chromosome facilitate the long-term storage of larger datum volume by using other DNA digital encoding and decoding programs.