Abstract：
Both infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) are the causative agents of acute and highly contagious diseases of juvenile salmonids, resulting in severe economic losses to these cold-water fish globally. There is an urgent need to explore antiviral agents against IHNV and IPNV due to the lack of commercially available vaccines and antiviral drugs. More importantly, the co-infection of IHNV and IPNV is prevalent in nature, which not only aggravates extensive damage to the salmonids but also poses challenges to its prevention and control. The antiviral effects of a crude polysaccharide derived from seaweed (CSP) on IHNV and IPNV were evaluated in this study separately. Furthermore, the underlying antiviral mechanisms of CSP to IHNV and IPNV were analyzed, respectively. The results showed that CSP possessed excellent safety and good ability to inhibit IHNV, IPNV, and their co-infection. CSP preferred to act at the early stage of viral infection. The antiviral mechanism of CSP on IHNV is possibly involved in preventing viral attachment and release, while in IPNV, it is involved in suppressing viral attachment, entry, and release. Taken together, the results of this study shed new light on developing novel agents against viral infection in salmonid fish.
摘要：
传染性造血系统坏死病毒（IHNV）和传染性胰腺坏死病毒（IPNV）都是幼年鲑鱼的急性和高度传染性疾病的病原体，导致全球这些冷水鱼遭受严重的经济损失。由于缺乏市售的疫苗和抗病毒药物,迫切需要探索针对IHNV和IPNV的抗病毒剂。更重要的是,IHNV和IPNV的共同感染在自然界中普遍存在，这不仅加剧了对鲑鱼的广泛破坏，而且对其预防和控制提出了挑战。本研究分别评估了海藻粗多糖（CSP）对IHNV和IPNV的抗病毒作用。此外，CSP对IHNV和IPNV的潜在抗病毒机制进行了分析，分别。结果表明，CSP具有优异的安全性和良好的抑制IHNV的能力，IPNV,以及它们的共同感染。CSP优选在病毒感染的早期起作用。CSP对IHNV的抗病毒机制可能涉及阻止病毒附着和释放，而在IPNV，它参与抑制病毒附着，条目，和释放。一起来看,这项研究的结果为开发抗鲑鱼病毒感染的新型药物提供了新的思路。
