Abstract：
Soybean leaf-associated gemygorvirus-1 (SlaGemV-1) is a CRESS-DNA virus classified in the family Genomoviridae, which causes hypovirulence and abolishes sclerotia formation in infected fungal pathogens under the family Sclerotiniaceae. To investigate the mechanisms involved in the induction of hypovirulence, RNA-Seq was compared between virus-free and SlaGemV-1-infected Sclerotinia sclerotiorum strain DK3. Overall, 4639 genes were differentially expressed, with 50.5% up regulated and 49.5% down regulated genes. GO enrichments suggest changes in integral membrane components and transmission electron microscopy images reveal virus-like particles localized near the inner cell membrane. Differential gene expression analysis focused on genes responsible for cell cycle and DNA replication and repair pathways, ubiquitin proteolysis, gene silencing, methylation, pathogenesis-related, sclerotial development, carbohydrate metabolism, and oxalic acid biosynthesis. Carbohydrate metabolism showed the most changes, with two glycoside hydrolase genes being the most down regulated by -2396.1- and -648.6-fold. Genes relating to pathogenesis showed consistent down regulation with the greatest being SsNep1, SsSSVP1, and Endo2 showing, -4555-, -14.7-, and -12.3-fold changes. The cell cycle and DNA replication/repair pathways were almost entirely up regulated including a putative cyclin and separase being up regulated 8.3- and 5.2-fold. The oxalate decarboxylase genes necessary for oxalic acid catabolism and oxalic acid precursor biosynthesis genes and its metabolism show down regulations of -17.2- and -12.1-fold changes. Sclerotial formation genes also appear differentially regulated including a melanin biosynthesis gene Pks1 and a sclerotia formation gene Sl2 with fold changes of 3.8 and -2.9.
摘要：
大豆叶相关的gemygorvirus-1（SlaGemV-1）是一种CRESS-DNA病毒，属于基因组病毒科，这导致低毒力并消除了核菌科下感染的真菌病原体中的菌核形成。为了研究诱导低毒力的机制，在无病毒和SlaGemV-1感染的核盘菌菌株DK3之间比较RNA-Seq。总的来说,4639个基因差异表达,50.5%上调基因和49.5%下调基因。GO富集表明整体膜成分的变化，透射电子显微镜图像显示位于内部细胞膜附近的病毒样颗粒。差异基因表达分析侧重于负责细胞周期和DNA复制和修复途径的基因。泛素蛋白水解，基因沉默,甲基化,发病机制相关，硬化发育，碳水化合物代谢，和草酸生物合成。碳水化合物代谢变化最多，其中两个糖苷水解酶基因被-2396.1-和-648.6-倍下调最多。与发病机制相关的基因显示一致的下调，其中最大的是SsNep1，SsSSVP1和Endo2，-4555-，-14.7-，和-12.3倍的变化。细胞周期和DNA复制/修复途径几乎完全上调，包括推定的细胞周期蛋白和分离酶上调8.3和5.2倍。草酸分解代谢所必需的草酸脱羧酶基因和草酸前体生物合成基因及其代谢显示出-17.2-和-12.1-倍变化的下调。硬化形成基因也表现出差异调节，包括黑色素生物合成基因Pks1和硬化形成基因Sl2，倍数变化为3.8和-2.9。
