Abstract：
The aquaculture industry is in need of sustainable fish feed to reduce the use of expensive and environmentally invasive wild-caught fish currently fed to many carnivorous species. The black soldier fly (BSF) has become a popular sustainable alternative protein source; however, the nutritional waste byproduct of BSF, frass, has not been extensively studied as a feed replacement in carnivorous species. This study evaluates the potential of BSF frass on the growth, body composition, and intestinal microbiome of the Florida pompano, Trachinotus carolinus. Four experimental diets were formulated containing different levels of frass, replacing plant-based carbohydrate sources. As a result of this study, the frass did not improve the growth performance, resulting in a lower specific growth rate and higher feed conversion rate. While the frass diets did not alter the body composition, the visceral somatic index (VSI) significantly increased compared to the control diet and the hepatosomatic index (HIS) was lowered. The microbiome analysis showed high variation among the diets, with the control diet having the most distinct consortia, which may have been driven by the increased levels of starch compared to frass diets. This study indicates that BSF frass may not be a suitable feed replacement for carnivorous pompano; however, frass could still potentially be a replacement feed for herbivore or detritivore fish and should be further studied.
摘要：
水产养殖业需要可持续的鱼饲料，以减少目前饲喂许多食肉物种的昂贵且具有环境入侵性的野生捕获鱼的使用。黑色士兵苍蝇（BSF）已成为一种流行的可持续替代蛋白质来源；然而，BSF的营养废物副产品，弗拉斯,尚未广泛研究作为食肉物种的饲料替代品。这项研究评估了BSF对生长的潜力，身体成分,和佛罗里达Pompano的肠道微生物组，CarolinusTrachinotuscarolinus.配制了四种实验饮食，其中含有不同水平的frass，替代植物性碳水化合物来源。作为这项研究的结果，弗拉斯没有改善增长业绩，导致较低的特定生长速率和较高的饲料转化率。虽然草率饮食没有改变身体成分，与对照饮食相比，内脏体细胞指数（VSI）显着增加，肝细胞指数（HIS）降低。微生物组分析显示，饮食之间的差异很大，对照饮食具有最明显的联盟，这可能是由于与弗拉斯饮食相比淀粉水平增加所致。这项研究表明，BSFfrass可能不是食肉pompano的合适饲料替代品；然而，Frass仍有可能成为食草动物或有害鱼类的替代饲料，应进一步研究。
