PDF(Pubmed)
Abstract：
UNASSIGNED: The immune system plays a vital role in cancer development and progression. Strategies mobilizing cytotoxic cells of the immune system to combat immunosuppression in cancer may help to improve the treatment response of patients. To this end, we aimed to characterize the anti-cancer effect of acute exercise, including the involvement of inflammatory signals.
UNASSIGNED: Twenty patients with early-stage prostate cancer (PCa) scheduled to undergo prostatectomy performed one bout of acute exercise consisting of a watt-max test and four high-intensity intervals. Natural Killer (NK), NKT-like and T cell phenotype, NK cell cytotoxic activity (NKCA), and NKCA per-cell against cell lines of leukemia (K562) and prostate cancer origin (LNCaP and PC-3) were assessed. Inflammatory markers (TNF-α, IL-6, and CRP) were measured in plasma.
UNASSIGNED: Exercise increased NK, NKT-like, and CD8 T cell concentration in the circulation. Furthermore, exercise shifted immune cells towards a mature and cytotoxic phenotype e.g., NK cells exhibited higher CD57 as well as lower NKG2A expression. NKT-like and CD8 cells exhibited elevated CD57, TIGIT and Granzyme-B expression. Exercise significantly improved NKCA against K562 (+16% [5%; 27%]; p = 0.002) and LNCaP (+24% [14%; 34%]; p < 0.001) but not PC-3. NKCA per NK cell decreased during exercise and increased 1-h post exercise compared to baseline in K562, LNCap, and PC-3 cell lines. Baseline IL-6 correlated with lymphocyte, monocyte and T cell concentration pre-exercise and inversely correlated with the fold-change of mobilized lymphocytes and CD8 T cells during exercise. Furthermore, baseline IL-6 and TNF-α inversely correlated with NKCA against PC-3 cells during exercise.
UNASSIGNED: Acute exercise mobilized cytotoxic immune cells and improved NKCA in patients with PCa whereas low-grade inflammation might impair the response. Whether the observed improvements impact long-term outcomes warrant further investigation.
UNASSIGNED: NCT03675529.
UNASSIGNED: Twenty patients with early-stage prostate cancer (PCa) scheduled to undergo prostatectomy performed one bout of acute exercise consisting of a watt-max test and four high-intensity intervals. Natural Killer (NK), NKT-like and T cell phenotype, NK cell cytotoxic activity (NKCA), and NKCA per-cell against cell lines of leukemia (K562) and prostate cancer origin (LNCaP and PC-3) were assessed. Inflammatory markers (TNF-α, IL-6, and CRP) were measured in plasma.
UNASSIGNED: Exercise increased NK, NKT-like, and CD8 T cell concentration in the circulation. Furthermore, exercise shifted immune cells towards a mature and cytotoxic phenotype e.g., NK cells exhibited higher CD57 as well as lower NKG2A expression. NKT-like and CD8 cells exhibited elevated CD57, TIGIT and Granzyme-B expression. Exercise significantly improved NKCA against K562 (+16% [5%; 27%]; p = 0.002) and LNCaP (+24% [14%; 34%]; p < 0.001) but not PC-3. NKCA per NK cell decreased during exercise and increased 1-h post exercise compared to baseline in K562, LNCap, and PC-3 cell lines. Baseline IL-6 correlated with lymphocyte, monocyte and T cell concentration pre-exercise and inversely correlated with the fold-change of mobilized lymphocytes and CD8 T cells during exercise. Furthermore, baseline IL-6 and TNF-α inversely correlated with NKCA against PC-3 cells during exercise.
UNASSIGNED: Acute exercise mobilized cytotoxic immune cells and improved NKCA in patients with PCa whereas low-grade inflammation might impair the response. Whether the observed improvements impact long-term outcomes warrant further investigation.
UNASSIGNED: NCT03675529.
摘要：
UNASSIGNED：免疫系统在癌症的发展和进展中起着至关重要的作用。动员免疫系统的细胞毒性细胞以对抗癌症中的免疫抑制的策略可能有助于改善患者的治疗反应。为此,我们旨在描述急性运动的抗癌作用，包括炎症信号的参与。
UNASSIGNED：计划接受前列腺切除术的20名早期前列腺癌（PCa）患者进行了一次急性运动，包括一次最大瓦特测试和四次高强度间隔。自然杀手（NK）NKT样和T细胞表型，NK细胞细胞毒活性（NKCA），和NKCA/细胞针对白血病细胞系(K562)和前列腺癌起源(LNCaP和PC-3)进行评估。炎症标志物(TNF-α，测量血浆中的IL-6和CRP）。
未经授权：运动增加NK，像NKT一样，和循环中的CD8T细胞浓度。此外，运动使免疫细胞转向成熟和细胞毒性表型，例如，NK细胞表现出较高的CD57以及较低的NKG2A表达。NKT样和CD8细胞表现出升高的CD57、TIGIT和颗粒酶B表达。运动显著改善了NKCA对K562(+16%[5%;27%];p=0.002)和LNCaP(+24%[14%;34%];p<0.001),但不是PC-3。与K562，LNCap的基线相比，运动期间每个NK细胞的NKCA减少，运动后1小时增加，和PC-3细胞系。基线IL-6与淋巴细胞相关，运动前单核细胞和T细胞浓度与运动过程中动员的淋巴细胞和CD8T细胞的倍数变化成反比。此外，在运动过程中，基线IL-6和TNF-α与NKCA对PC-3细胞呈负相关。
UNASSIGNED：急性运动可动员PCa患者的细胞毒性免疫细胞并改善NKCA，而低度炎症可能会损害反应。观察到的改善是否影响长期结果值得进一步调查。
未经评估：NCT03675529。
UNASSIGNED：计划接受前列腺切除术的20名早期前列腺癌（PCa）患者进行了一次急性运动，包括一次最大瓦特测试和四次高强度间隔。自然杀手（NK）NKT样和T细胞表型，NK细胞细胞毒活性（NKCA），和NKCA/细胞针对白血病细胞系(K562)和前列腺癌起源(LNCaP和PC-3)进行评估。炎症标志物(TNF-α，测量血浆中的IL-6和CRP）。
未经授权：运动增加NK，像NKT一样，和循环中的CD8T细胞浓度。此外，运动使免疫细胞转向成熟和细胞毒性表型，例如，NK细胞表现出较高的CD57以及较低的NKG2A表达。NKT样和CD8细胞表现出升高的CD57、TIGIT和颗粒酶B表达。运动显著改善了NKCA对K562(+16%[5%;27%];p=0.002)和LNCaP(+24%[14%;34%];p<0.001),但不是PC-3。与K562，LNCap的基线相比，运动期间每个NK细胞的NKCA减少，运动后1小时增加，和PC-3细胞系。基线IL-6与淋巴细胞相关，运动前单核细胞和T细胞浓度与运动过程中动员的淋巴细胞和CD8T细胞的倍数变化成反比。此外，在运动过程中，基线IL-6和TNF-α与NKCA对PC-3细胞呈负相关。
UNASSIGNED：急性运动可动员PCa患者的细胞毒性免疫细胞并改善NKCA，而低度炎症可能会损害反应。观察到的改善是否影响长期结果值得进一步调查。
未经评估：NCT03675529。
