UNASSIGNED: Twenty patients with early-stage prostate cancer (PCa) scheduled to undergo prostatectomy performed one bout of acute exercise consisting of a watt-max test and four high-intensity intervals. Natural Killer (NK), NKT-like and T cell phenotype, NK cell cytotoxic activity (NKCA), and NKCA per-cell against cell lines of leukemia (K562) and prostate cancer origin (LNCaP and PC-3) were assessed. Inflammatory markers (TNF-α, IL-6, and CRP) were measured in plasma.
UNASSIGNED: Exercise increased NK, NKT-like, and CD8 T cell concentration in the circulation. Furthermore, exercise shifted immune cells towards a mature and cytotoxic phenotype e.g., NK cells exhibited higher CD57 as well as lower NKG2A expression. NKT-like and CD8 cells exhibited elevated CD57, TIGIT and Granzyme-B expression. Exercise significantly improved NKCA against K562 (+16% [5%; 27%]; p = 0.002) and LNCaP (+24% [14%; 34%]; p < 0.001) but not PC-3. NKCA per NK cell decreased during exercise and increased 1-h post exercise compared to baseline in K562, LNCap, and PC-3 cell lines. Baseline IL-6 correlated with lymphocyte, monocyte and T cell concentration pre-exercise and inversely correlated with the fold-change of mobilized lymphocytes and CD8 T cells during exercise. Furthermore, baseline IL-6 and TNF-α inversely correlated with NKCA against PC-3 cells during exercise.
UNASSIGNED: Acute exercise mobilized cytotoxic immune cells and improved NKCA in patients with PCa whereas low-grade inflammation might impair the response. Whether the observed improvements impact long-term outcomes warrant further investigation.
UNASSIGNED: NCT03675529.
UNASSIGNED:计划接受前列腺切除术的20名早期前列腺癌(PCa)患者进行了一次急性运动,包括一次最大瓦特测试和四次高强度间隔。自然杀手(NK)NKT样和T细胞表型,NK细胞细胞毒活性(NKCA),和NKCA/细胞针对白血病细胞系(K562)和前列腺癌起源(LNCaP和PC-3)进行评估。炎症标志物(TNF-α,测量血浆中的IL-6和CRP)。
未经授权:运动增加NK,像NKT一样,和循环中的CD8T细胞浓度。此外,运动使免疫细胞转向成熟和细胞毒性表型,例如,NK细胞表现出较高的CD57以及较低的NKG2A表达。NKT样和CD8细胞表现出升高的CD57、TIGIT和颗粒酶B表达。运动显著改善了NKCA对K562(+16%[5%;27%];p=0.002)和LNCaP(+24%[14%;34%];p<0.001),但不是PC-3。与K562,LNCap的基线相比,运动期间每个NK细胞的NKCA减少,运动后1小时增加,和PC-3细胞系。基线IL-6与淋巴细胞相关,运动前单核细胞和T细胞浓度与运动过程中动员的淋巴细胞和CD8T细胞的倍数变化成反比。此外,在运动过程中,基线IL-6和TNF-α与NKCA对PC-3细胞呈负相关。
UNASSIGNED:急性运动可动员PCa患者的细胞毒性免疫细胞并改善NKCA,而低度炎症可能会损害反应。观察到的改善是否影响长期结果值得进一步调查。
未经评估:NCT03675529。