Abstract：
The underlying mechanism of the role of mitochondria in color changing of tilapia fillet during 0-4 d storage is not completely clear. A total of 209 differentially significant expressed proteins (DSEPs) were identified by using label-free mitochondrial proteomics, with 56 proteins up-regulated in T2 and 61 proteins (up-regulated) in T3. Protein-Protein interaction reveled proteins which participate in TCA cycles (Citrate synthase (cs)), Oxidoreductase (Malate dehydrogenase (mdh1, mdh2), Succinyl-CoA (Oxct1), Hydroxyacyl-coenzyme a dehydrogenase (hadh), Dehydrogenase/reductase (SDR family) member 1 (dhrs1)) interacted strongly with each other. In turn, they can increase the level of mitochondrial respiration and mitochondrial function, leading to color changing of tilapia fillet. The heat shock 60kD protein 1 (chaperonin, hspd1) interacted with metabolic enzymes (cs and mdh2) and had important effects on color. These results could help researchers better understand the color changing mechanism on the surface of tilapia fillet during the storage.
摘要：
线粒体在罗非鱼片0-4d贮藏过程中颜色变化的作用机制尚不完全清楚。通过无标记线粒体蛋白质组学鉴定了209种差异显著表达蛋白(DSEP),其中56种蛋白质在T2中上调，61种蛋白质在T3中上调。蛋白质-蛋白质相互作用揭示了参与TCA循环的蛋白质(柠檬酸合酶(cs))，氧化还原酶（苹果酸脱氢酶（mdh1，mdh2），琥珀酰辅酶A（Oxct1），羟酰辅酶a脱氢酶（hadh），脱氢酶/还原酶（SDR家族）成员1（dhrs1））彼此强烈相互作用。反过来,它们可以增加线粒体呼吸和线粒体功能的水平，导致罗非鱼片变色。热休克60kD蛋白1(伴奏蛋白，hspd1）与代谢酶（cs和mdh2）相互作用，对颜色有重要影响。这些结果可以帮助研究人员更好地了解罗非鱼片在贮藏过程中表面的颜色变化机理。
