Abstract：
In this study, we explored the suspected pathogens of chronic periodontitis at different stages of occurrence and development. We collected 100 gingival crevicular fluid samples, 27, 27, and 26 from patients with mild, moderate, and severe chronic periodontitis, respectively, and 20 from healthy individuals. Pathogens were detected using a 16S rRNA metagenomic approach. Quantitative Insights in Microbial Ecology, Mothur, and other software were used to analyze the original data, draw relative abundance histograms and heat maps, and calculate flora abundance and diversity indexes. We identified 429 operational taxonomic units, covering 13 phyla, 20 classes, 32 orders, 66 families, and 123 genera from the four groups of samples. Each group showed microbial diversity, and the number of new species of bacterial flora in the gingival crevicular fluid samples gradually increased from the healthy to the severe chronic periodontitis group. There was a significant difference in the relative abundance of the core flora at the phylum, class, order, family, and genus classification levels. Our data indicated a certain correlation between the changes in the subgingival microbial structure and the occurrence and development of chronic periodontitis, which might be able to provide a reference for the diagnosis, treatment and prevention of chronic periodontitis.
摘要：
在这项研究中,我们探讨了慢性牙周炎在发生和发展不同阶段的可疑病原体。我们采集了100个龈沟液样本,27、27和26来自轻度患者，中度,和严重的慢性牙周炎,分别,20个来自健康个体。使用16SrRNA宏基因组方法检测病原体。微生物生态学中的定量见解，莫瑟,和其他软件用于分析原始数据，绘制相对丰度直方图和热图，并计算植物区系丰度和多样性指数。我们确定了429个操作分类单位，覆盖13门，20班,32个订单,66个家庭,和来自四组样品的123个属。各组均表现出微生物多样性,从健康到重度慢性牙周炎组，龈沟液中细菌菌群的新物种数量逐渐增加。门核心区系的相对丰度存在显著差异,类,订单,家庭,和属分类水平。我们的数据表明，龈下微生物结构的变化与慢性牙周炎的发生和发展之间存在一定的相关性。这也许可以为诊断提供参考，治疗和预防慢性牙周炎。
