Abstract：
To explore the relationship between the mitfa gene and intestinal microbiota, the 16S rRNA gene amplicon sequencing was performed to compare the intestinal microbiota composition of the mitfa knockout zebrafish line (CKO group) and the wild-type zebrafish (WT group) in this study. The results showed that the Fusobacteria and Firmicutes were significantly decreased and the Dependentiae and Patescibacteria were significantly increased in the CKO group at the phylum level. Furthermore, the relative abundance of Citrobacter, Gordonia, Mesorhizobium, Legionella, and Bradyrhizobium were extremely higher in the CKO group, whereas the other four genera Nocardia, Pannonibacter, Shinella, and Cetobacterium were significantly declined in the CKO group at the genus level. Due to these changed intestinal microbiota appear to be related to lipid metabolism and immunity, eight lipid metabolism-related genes and nine inflammation-related genes were detected in the intestinal. The results showed that the expression levels of these genes were significant differences between the CKO and WT group. These results indicated that the deletion of mitfa can affect the expression levels of immune and metabolism-related genes, and causing changes in the composition of the intestinal microbiota.
摘要：
探讨mitfa基因与肠道菌群的关系,在这项研究中,我们进行了16SrRNA基因扩增子测序,以比较mitfa敲除斑马鱼品系(CKO组)和野生型斑马鱼(WT组)的肠道微生物组成.结果表明，在门水平上，CKO组的Fusobacteria和Firmicutes显着减少，而Dependentiae和Patescible显着增加。此外，柠檬酸杆菌的相对丰度，Gordonia,中根瘤菌,军团菌,CKO组中根瘤菌的含量极高，而其他四个属诺卡氏菌，潘纳杆菌,Shinella,在属水平上，CKO组中的Cetobacterium显着下降。由于这些改变的肠道菌群似乎与脂质代谢和免疫有关，在肠道中检测到8个脂质代谢相关基因和9个炎症相关基因。结果表明，这些基因的表达水平在CKO和WT组之间存在显着差异。这些结果表明mitfa的缺失可以影响免疫和代谢相关基因的表达水平，并引起肠道微生物群组成的变化。
