Abstract：
One of organic ultraviolet (UV) filters, Octocrylene (OCL), is mainly used in various cosmetic products, which is being frequently detected in soil, sediment, aquatic systems and food chain. There is evidence confirmed the reproductive toxicity of OCL in Japanese medaka. However, less was known about the effects of OCL exposure on oocyte quality. Here, we investigated the impacts of OCL on mouse oocyte maturation and quality by exposing oocytes to OCL in vitro at concentrations of 8, 22, 30, 40 and 50 nM. The results showed that OCL markedly reduced mouse oocyte germinal vesicle breakdown (GVBD) at 50 nM and polar body extrusion (PBE) rates at 40 and 50 nM. OCL exposure further disrupted spindle assembly and chromosome alignment, finally inducing aneuploid. Mitochondrial function was also damaged by OCL exposure, leading to ROS overproduction and apoptosis in oocytes. Moreover, OCL treatment impaired the distribution of cortical granules and sperm binding ability of oocytes. In summary, these data demonstrated that OCL could disturb the oocyte meiotic maturation and reduce oocyte quality.
摘要：
有机紫外线（UV）过滤器之一，八氯丙烯(OCL),主要用于各种化妆品，在土壤中经常被检测到，沉积物,水生系统和食物链。有证据证实了OCL在日本medaka中的生殖毒性。然而,关于OCL暴露对卵母细胞质量的影响知之甚少。这里,我们通过将卵母细胞暴露于体外浓度为8、22、30、40和50nM的OCL，研究了OCL对小鼠卵母细胞成熟和质量的影响。结果表明，OCL显着降低了50nM的小鼠卵母细胞胚泡破裂（GVBD）和40和50nM的极体挤压（PBE）速率。OCL暴露进一步破坏纺锤体组装和染色体排列，最后诱导非整倍体。OCL暴露也损害了线粒体功能，导致卵母细胞中ROS过量产生和凋亡。此外，OCL治疗损害了皮质颗粒的分布和卵母细胞的精子结合能力。总之,这些数据表明OCL可以干扰卵母细胞减数分裂成熟并降低卵母细胞质量。
