Abstract：
The molecular mechanism underlying brain regeneration in vertebrates remains elusive. We performed spatial enhanced resolution omics sequencing (Stereo-seq) to capture spatially resolved single-cell transcriptomes of axolotl telencephalon sections during development and regeneration. Annotated cell types exhibited distinct spatial distribution, molecular features, and functions. We identified an injury-induced ependymoglial cell cluster at the wound site as a progenitor cell population for the potential replenishment of lost neurons, through a cell state transition process resembling neurogenesis during development. Transcriptome comparisons indicated that these induced cells may originate from local resident ependymoglial cells. We further uncovered spatially defined neurons at the lesion site that may regress to an immature neuron-like state. Our work establishes spatial transcriptome profiles of an anamniote tetrapod brain and decodes potential neurogenesis from ependymoglial cells for development and regeneration, thus providing mechanistic insights into vertebrate brain regeneration.
摘要：
脊椎动物脑再生的分子机制仍然难以捉摸。我们进行了空间增强分辨率组学测序（Stereo-seq），以捕获发育和再生过程中轴突端脑切片的空间分辨单细胞转录组。带注释的细胞类型表现出明显的空间分布，分子特征，和功能。我们确定了创伤部位的损伤诱导的室管膜胶质细胞簇作为祖细胞群，用于潜在补充丢失的神经元。通过类似于发育过程中神经发生的细胞状态转变过程。转录组比较表明，这些诱导的细胞可能起源于局部驻留的室管膜胶质细胞。我们进一步发现了病变部位的空间定义的神经元，这些神经元可能会退化为未成熟的神经元样状态。我们的工作建立了羊水四足动物大脑的空间转录组概况，并从室管膜胶质细胞中解码潜在的神经发生，用于发育和再生。从而提供了脊椎动物大脑再生的机械见解。
