Abstract：
The effects of a fructose-rich diet and chronic stress on copper metabolism in the kidneys are still understudied. We investigated whether fructose and/or chronic unpredictable stress modulate copper metabolism in a way that affects redox homeostasis, thus contributing to progression of metabolic disturbances in the kidney. We determined protein level of copper transporters, chaperones, and cuproenzymes including cytochrome c oxidase, as well as antioxidant enzymes function in the kidneys of male Wistar rats subjected to 20% liquid fructose supplementation and/or chronic stress. Liquid fructose supplementation increased level of copper chaperone of superoxide dismutase and decreased metallothionein level, while rendering the level of copper importer and copper chaperones involved in copper delivery to mitochondria and trans Golgi network unaffected. Stress had no effect on renal copper metabolism. The activity and expression of renal antioxidant enzymes remained unaltered in all experimental groups. In conclusion, fructose, independently of stress, decreased renal copper level, and modulated renal copper metabolism as to preserve vital cellular function including mitochondrial energy production and antioxidative defense, at the expense of intracellular copper storage.
摘要：
富含果糖的饮食和慢性压力对肾脏铜代谢的影响仍未得到充分研究。我们调查了果糖和/或慢性不可预测的应激是否以影响氧化还原稳态的方式调节铜代谢。从而导致肾脏代谢紊乱的进展。我们确定了铜转运蛋白的蛋白质水平，监护人,和包括细胞色素C氧化酶在内的铜酶，以及抗氧化酶在接受20％液体果糖补充和/或慢性应激的雄性Wistar大鼠的肾脏中起作用。补充液体果糖可增加超氧化物歧化酶的铜伴侣水平，并降低金属硫蛋白水平，同时使铜向线粒体和跨高尔基网络的铜进口商和铜伴侣的水平不受影响。应激对肾铜代谢无影响。在所有实验组中，肾脏抗氧化酶的活性和表达保持不变。总之,果糖,独立于压力，肾铜水平降低，并调节肾脏铜代谢，以保持重要的细胞功能，包括线粒体能量产生和抗氧化防御，以细胞内铜储存为代价。
