Abstract：
Campylobacter is recognised as one of the most important foodborne bacteria, with a worldwide health and socioeconomic impact. This bacterium is one of the most important zoonotic players in poultry, where efficient and fast detection methods are required. Current official culture methods for Campylobacter enumeration in poultry usually include >44 h of culture and >72 h for identification, thus requiring at least five working shifts (ISO/TS 10272-2:2017). Here, we have assembled a portable sequencing kit composed of the Bento Lab and the MinION and developed a workflow for on-site farm use that is able to detect and report the presence of Campylobacter from caecal samples in less than five hours from sampling time, as well as the relationship of Campylobacter with other caecal microbes. Beyond that, our workflow may offer a cost-effective and practical method of microbiologically monitoring poultry at the farm. These results would demonstrate the possibility of carrying out rapid on-site screening to monitor the health status of the poultry farm/flock during the production chain.
摘要：
弯曲杆菌被认为是最重要的食源性细菌之一，具有全球健康和社会经济影响。这种细菌是家禽中最重要的人畜共患细菌之一，其中需要高效和快速的检测方法。目前家禽弯曲杆菌计数的官方培养方法通常包括培养44小时和鉴定72小时，因此需要至少五个工作班次(ISO/TS10272-2:2017)。这里,我们已经组装了一个由Bento实验室和MinION组成的便携式测序试剂盒，并开发了一个现场农场使用的工作流程，能够在采样时间不到5小时的时间内检测和报告盲肠样品中弯曲杆菌的存在，以及弯曲杆菌与其他盲肠微生物的关系。除此之外，我们的工作流程可能会提供一种经济有效且实用的方法来对农场的家禽进行微生物监测.这些结果将证明在生产链中进行快速现场筛查以监测家禽养殖场/群的健康状况的可能性。
