Abstract：
Eugenol is the main constituent of clove extract. It is a remarkably versatile molecule incorporated as a functional ingredient in several food products and widely applied in the pharmaceutical industry. Men consume natural products enriched with eugenol for treating sexual disorders and using as aphrodisiacs. Nevertheless, there is no information about the impact of eugenol intake on the reproductive parameters of healthy males. Therefore, we provided 10, 20, and 40 mg kg-1 pure eugenol to adult Wistar rats for 60 days. Testis, epididymis, and spermatozoa were analyzed under microscopic, biochemical, and functional approaches. This phenolic compound did not alter testicular and epididymal biometry and microscopy. However, 20 and 40 mg kg-1 eugenol reduced serum testosterone levels. The highest dose altered lactate and glucose concentrations in the epididymis. All the eugenol concentrations diminished CAT activity and MDA levels in the testis and increased FRAP and CAT activity in the epididymis. Epididymal sperm from rats receiving 10, 20, and 40 mg kg-1 eugenol presented high Ca2+ ATPase activity and low motility. In conclusion, eugenol at low and high doses negatively impacted the competence of epididymal sperm and modified oxidative parameters in male organs, with no influence on their microscopy.
摘要：
丁香酚是丁香提取物的主要成分。它是一种非常通用的分子，作为功能成分掺入几种食品中，并广泛应用于制药工业。男性食用富含丁香酚的天然产品，用于治疗性疾病和用作壮阳药。然而，没有关于丁香酚摄入对健康男性生殖参数影响的信息。因此,我们为成年Wistar大鼠提供了10,20和40mgkg-1纯丁香酚，共60天。睾丸,附睾,和精子在显微镜下分析，生物化学，和功能性方法。这种酚类化合物不会改变睾丸和附睾生物特征和显微镜检查。然而,20和40mgkg-1丁香酚可降低血清睾酮水平。最高剂量改变了附睾中的乳酸和葡萄糖浓度。所有丁香酚浓度均降低了睾丸中的CAT活性和MDA水平，并增加了附睾中的FRAP和CAT活性。接受10、20和40mgkg-1丁香酚的大鼠附睾精子表现出高Ca2ATPase活性和低运动性。总之,低剂量和高剂量的丁香酚对男性器官附睾精子的能力和氧化参数的改变产生负面影响，对他们的显微镜没有影响。
