Abstract：
Electric fields (EFs) are thought to play a decisive role in wound healing. However, most studies focused on the effects of EF on single species of cells in vitro. Here, we aimed to investigate the coordination function of EFs on wound healing. Using a bamamini pig whole-layer wound model, we further evaluated the potential of EFs as a treatment modality by applying continuous and stable EF to the wound, and we found that EF promoted wound contraction and re-epithelialization in vivo, which accelerated wound healing. In vitro, we found that EFs significantly promoted the collective migration of HaCaT cells, guided HSF cells rearrangement, and promoted collagen secretion and myofibroblast transformation, and the electrotaxis of HaCaT cells was significantly enhanced on the collagen substrate and F-actin polarization at the leading edge of the cells was more pronounced. Overall, we determined that EF promotes wound contraction by promoting myofibrillar transformation, while accelerating the formation of collagen substrates, and the substrates could provide a good basis for electric field-guided re-epithelialization. EF may promote wound healing in multiple dimensions interaction and coordinate the whole process of wound healing. These findings provide support for the continued development of EF for wound treatment applications.
摘要：
电场(EF)被认为在伤口愈合中起决定性作用。然而,大多数研究集中在EF对体外单个细胞的影响。这里,我们旨在研究EFs对伤口愈合的协调功能。使用Bamamini猪全层伤口模型，我们通过对伤口施加连续和稳定的EF来进一步评估EF作为治疗方式的潜力，我们发现EF在体内促进伤口收缩和上皮再生，加速伤口愈合。体外，我们发现EF显著促进HaCaT细胞的集体迁移，引导的HSF细胞重排，并促进胶原蛋白分泌和肌成纤维细胞转化，而且HaCaT细胞在胶原底物上的电滑行显著增强，细胞前缘的F-肌动蛋白极化更加明显。总的来说,我们确定EF通过促进肌原纤维转化来促进伤口收缩，在加速胶原蛋白底物形成的同时，基底可以为电场引导的上皮再形成提供良好的基础。EF可以促进伤口愈合，在多个维度相互作用，协调伤口愈合的整个过程。这些发现为用于伤口治疗应用的EF的持续发展提供了支持。
