PDF(Pubmed)
Abstract：
UNASSIGNED: The consumption of lycopene-rich foods may lower cardiovascular disease (CVD) risk. Lycopene circulates in the blood bound to lipoproteins, including high-density lipoproteins (HDLs). Preliminary data from our group showed that increased consumption of tomato-based food or lycopene supplement in middle-aged subjects led to functional changes to HDL\'s sub-fractions, HDL2 and HDL3. These changes were also associated with a decrease in serum amyloid A (SAA), potentially enhancing their anti-atherogenic properties.
UNASSIGNED: We carried out a comprehensive randomized controlled intervention trial with healthy middle-aged volunteers to assess whether the consumption of tomato-based foods or lycopene supplements affects HDL functionality and associated inflammatory markers, and lipoprotein subfractions size and distribution.
UNASSIGNED: Volunteers (225, aged 40-65 years) were randomly assigned to one of three dietary intervention groups and asked to consume a control diet (low in tomato-based foods, <10 mg lycopene/week), a lycopene-rich diet (224-350 mg lycopene/week), or the control diet with a lycopene supplement (70 mg lycopene/week). HDL2 and HDL3 were isolated by ultracentrifugation. Compliance was monitored by assessing lycopene concentration in serum. Systemic and HDL-associated inflammation was assessed by measuring SAA concentrations. HDL functionality was determined by monitoring paraoxonase-1 (PON-1), cholesteryl ester transfer protein (CETP), and lecithin cholesterol acyltransferase (LCAT) activities. The lipoprotein subfractions profile was assessed by NMR.
UNASSIGNED: Lycopene in serum and HDL significantly increased following consumption of both the high tomato diet and lycopene supplement (p ≤ 0.001 for both). Lycopene, either as a tomato-rich food or a supplement, enhanced both serum- and HDL3-PON-1 activities (p ≤ 0.001 and p = 0.036, respectively), while significantly reducing HDL3-SAA-related inflammation (p = 0.001). Lycopene supplement also significantly increased HDL3-LCAT activity (p = 0.05), and reduced the activity of both HDL2- and HDL3-CETP (p = 0.005 and p = 0.002, respectively). These changes were not associated with changes in the subclasses distribution for all lipoprotein fractions or the size of lipoprotein subclasses.
UNASSIGNED: Our results showed that dietary lycopene can significantly enhance HDL functionality, without associated changes in particle size and distribution, by modulating the activity of HDL-associated enzymes. Concomitantly, dietary lycopene significantly decreased serum- and HDL3-associated SAA, confirming that SAA may represent a sensitive inflammatory biomarker to dietary change.
UNASSIGNED: (https://www.isrctn.com), ISRCTN34203810.
UNASSIGNED: We carried out a comprehensive randomized controlled intervention trial with healthy middle-aged volunteers to assess whether the consumption of tomato-based foods or lycopene supplements affects HDL functionality and associated inflammatory markers, and lipoprotein subfractions size and distribution.
UNASSIGNED: Volunteers (225, aged 40-65 years) were randomly assigned to one of three dietary intervention groups and asked to consume a control diet (low in tomato-based foods, <10 mg lycopene/week), a lycopene-rich diet (224-350 mg lycopene/week), or the control diet with a lycopene supplement (70 mg lycopene/week). HDL2 and HDL3 were isolated by ultracentrifugation. Compliance was monitored by assessing lycopene concentration in serum. Systemic and HDL-associated inflammation was assessed by measuring SAA concentrations. HDL functionality was determined by monitoring paraoxonase-1 (PON-1), cholesteryl ester transfer protein (CETP), and lecithin cholesterol acyltransferase (LCAT) activities. The lipoprotein subfractions profile was assessed by NMR.
UNASSIGNED: Lycopene in serum and HDL significantly increased following consumption of both the high tomato diet and lycopene supplement (p ≤ 0.001 for both). Lycopene, either as a tomato-rich food or a supplement, enhanced both serum- and HDL3-PON-1 activities (p ≤ 0.001 and p = 0.036, respectively), while significantly reducing HDL3-SAA-related inflammation (p = 0.001). Lycopene supplement also significantly increased HDL3-LCAT activity (p = 0.05), and reduced the activity of both HDL2- and HDL3-CETP (p = 0.005 and p = 0.002, respectively). These changes were not associated with changes in the subclasses distribution for all lipoprotein fractions or the size of lipoprotein subclasses.
UNASSIGNED: Our results showed that dietary lycopene can significantly enhance HDL functionality, without associated changes in particle size and distribution, by modulating the activity of HDL-associated enzymes. Concomitantly, dietary lycopene significantly decreased serum- and HDL3-associated SAA, confirming that SAA may represent a sensitive inflammatory biomarker to dietary change.
UNASSIGNED: (https://www.isrctn.com), ISRCTN34203810.
摘要：
■食用富含番茄红素的食物可能会降低心血管疾病（CVD）的风险。番茄红素在与脂蛋白结合的血液中循环，包括高密度脂蛋白（HDLs）。我们小组的初步数据显示，中年受试者食用以番茄为基础的食物或补充番茄红素的增加导致高密度脂蛋白亚组分的功能变化，HDL2和HDL3。这些变化也与血清淀粉样蛋白A（SAA）的减少有关，可能增强其抗动脉粥样硬化的特性。
我们对健康的中年志愿者进行了一项全面的随机对照干预试验，以评估食用以番茄为基础的食物或番茄红素补充剂是否会影响HDL功能和相关的炎症标志物。和脂蛋白亚组分的大小和分布。
■志愿者（225名，年龄40-65岁）被随机分配到三个饮食干预组之一，并被要求食用对照饮食（低番茄基食物，<10毫克番茄红素/周），富含番茄红素的饮食（224-350毫克番茄红素/周），或控制饮食与番茄红素补充剂（70毫克番茄红素/周）。通过超速离心分离HDL2和HDL3。通过评估血清中的番茄红素浓度来监测依从性。通过测量SAA浓度来评估全身和HDL相关的炎症。HDL功能是通过监测对氧磷酶-1（PON-1），胆固醇酯转移蛋白（CETP），和卵磷脂胆固醇酰基转移酶（LCAT）活性。通过NMR评估脂蛋白亚组分谱。
■食用高番茄饮食和番茄红素补充剂后，血清和HDL中的番茄红素显着增加（两者的p≤0.001）。番茄红素,作为富含番茄的食物或补充剂，血清和HDL3-PON-1活性均增强（分别为p≤0.001和p=0.036），同时显着减少HDL3-SAA相关的炎症（p=0.001）。补充番茄红素也显著增加HDL3-LCAT活性（p=0.05），并降低HDL2-和HDL3-CETP的活性（分别为p=0.005和p=0.002）。这些变化与所有脂蛋白组分的亚类分布或脂蛋白亚类大小的变化无关。
■我们的结果表明，膳食番茄红素可以显着增强HDL功能，没有相关的颗粒大小和分布的变化，通过调节HDL相关酶的活性。同时，饮食番茄红素显着降低血清和HDL3相关的SAA，证实SAA可能代表饮食变化的敏感炎症生物标志物。
■(https://www.isrctn.com),ISRCTN34203810。
我们对健康的中年志愿者进行了一项全面的随机对照干预试验，以评估食用以番茄为基础的食物或番茄红素补充剂是否会影响HDL功能和相关的炎症标志物。和脂蛋白亚组分的大小和分布。
■志愿者（225名，年龄40-65岁）被随机分配到三个饮食干预组之一，并被要求食用对照饮食（低番茄基食物，<10毫克番茄红素/周），富含番茄红素的饮食（224-350毫克番茄红素/周），或控制饮食与番茄红素补充剂（70毫克番茄红素/周）。通过超速离心分离HDL2和HDL3。通过评估血清中的番茄红素浓度来监测依从性。通过测量SAA浓度来评估全身和HDL相关的炎症。HDL功能是通过监测对氧磷酶-1（PON-1），胆固醇酯转移蛋白（CETP），和卵磷脂胆固醇酰基转移酶（LCAT）活性。通过NMR评估脂蛋白亚组分谱。
■食用高番茄饮食和番茄红素补充剂后，血清和HDL中的番茄红素显着增加（两者的p≤0.001）。番茄红素,作为富含番茄的食物或补充剂，血清和HDL3-PON-1活性均增强（分别为p≤0.001和p=0.036），同时显着减少HDL3-SAA相关的炎症（p=0.001）。补充番茄红素也显著增加HDL3-LCAT活性（p=0.05），并降低HDL2-和HDL3-CETP的活性（分别为p=0.005和p=0.002）。这些变化与所有脂蛋白组分的亚类分布或脂蛋白亚类大小的变化无关。
■我们的结果表明，膳食番茄红素可以显着增强HDL功能，没有相关的颗粒大小和分布的变化，通过调节HDL相关酶的活性。同时，饮食番茄红素显着降低血清和HDL3相关的SAA，证实SAA可能代表饮食变化的敏感炎症生物标志物。
■(https://www.isrctn.com),ISRCTN34203810。
