Abstract：
OBJECTIVE: The cell cycle-related proteins cyclin B1 (CCNB1) and cyclin B2 (CCNB2) are potentially involved in the underlying mechanisms of psoriasis. The present study aimed to explore this possibility using bioinformatics approaches.
METHODS: CCNB1 and CCNB2 protein levels were evaluated in 14 psoriasis patients and five healthy controls by enzyme-linked immunosorbent assays, and their mRNA levels were evaluated using data from four publicly available datasets (GSE53552, GSE41664, GSE14905, and GSE13355). Comparison of high- and low-expressing groups were performed to reveal CCNB1- and CCNB2-related differentially expressed genes, which were then assessed based on gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses. Correlation analyses between CCNB1 and CCNB2 levels and immune infiltration, as well as typical targets of psoriasis, were also performed.
RESULTS: Overall, 12 CCNB1 and CCNB2 common immune-related targets potentially involved in psoriasis were identified. These could regulate the cell cycle of through multiple pathways. In addition, CCNB1 and CCNB2 were found to potentially support the release of key molecular targets of psoriasis through the regulation of mast cell activation and macrophage polarization.
CONCLUSIONS: These findings suggest that CCNB1 and CCNB2 may represent valuable molecular biomarkers of psoriasis, contributing to its onset and progression.
METHODS: CCNB1 and CCNB2 protein levels were evaluated in 14 psoriasis patients and five healthy controls by enzyme-linked immunosorbent assays, and their mRNA levels were evaluated using data from four publicly available datasets (GSE53552, GSE41664, GSE14905, and GSE13355). Comparison of high- and low-expressing groups were performed to reveal CCNB1- and CCNB2-related differentially expressed genes, which were then assessed based on gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses. Correlation analyses between CCNB1 and CCNB2 levels and immune infiltration, as well as typical targets of psoriasis, were also performed.
RESULTS: Overall, 12 CCNB1 and CCNB2 common immune-related targets potentially involved in psoriasis were identified. These could regulate the cell cycle of through multiple pathways. In addition, CCNB1 and CCNB2 were found to potentially support the release of key molecular targets of psoriasis through the regulation of mast cell activation and macrophage polarization.
CONCLUSIONS: These findings suggest that CCNB1 and CCNB2 may represent valuable molecular biomarkers of psoriasis, contributing to its onset and progression.
摘要：
目的：细胞周期相关蛋白cyclinB1(CCNB1)和cyclinB2(CCNB2)可能参与银屑病的潜在机制。本研究旨在利用生物信息学方法探索这种可能性。
方法：通过酶联免疫吸附试验评估了14例银屑病患者和5例健康对照者的CCNB1和CCNB2蛋白水平，使用来自四个公开数据集(GSE53552,GSE41664,GSE14905和GSE13355)的数据评估了它们的mRNA水平.比较高和低表达组，以揭示CCNB1-和CCNB2相关的差异表达基因，然后根据基因本体论和京都基因百科全书和基因组途径分析进行评估。CCNB1和CCNB2水平与免疫浸润的相关性分析,以及牛皮癣的典型目标，也表演了。
结果：总体而言，确定了12种可能与银屑病有关的CCNB1和CCNB2常见免疫相关靶标。这些可以通过多种途径调节细胞周期。此外,发现CCNB1和CCNB2可能通过调节肥大细胞活化和巨噬细胞极化来支持银屑病关键分子靶标的释放。
结论：这些研究结果表明，CCNB1和CCNB2可能代表银屑病的有价值的分子生物标志物，有助于其发作和进展。
方法：通过酶联免疫吸附试验评估了14例银屑病患者和5例健康对照者的CCNB1和CCNB2蛋白水平，使用来自四个公开数据集(GSE53552,GSE41664,GSE14905和GSE13355)的数据评估了它们的mRNA水平.比较高和低表达组，以揭示CCNB1-和CCNB2相关的差异表达基因，然后根据基因本体论和京都基因百科全书和基因组途径分析进行评估。CCNB1和CCNB2水平与免疫浸润的相关性分析,以及牛皮癣的典型目标，也表演了。
结果：总体而言，确定了12种可能与银屑病有关的CCNB1和CCNB2常见免疫相关靶标。这些可以通过多种途径调节细胞周期。此外,发现CCNB1和CCNB2可能通过调节肥大细胞活化和巨噬细胞极化来支持银屑病关键分子靶标的释放。
结论：这些研究结果表明，CCNB1和CCNB2可能代表银屑病的有价值的分子生物标志物，有助于其发作和进展。
