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Abstract：
UNASSIGNED: As the treatment target, the imaging information and histologic characteristics of the thrombus may differ according to the stroke subtype. This study aimed to provide the correlative study of stroke etiology with the non-contrast CT, and histological composition of retrieved clots in acute ischemic stroke (AIS).
UNASSIGNED: A total of 94 patients with AIS who underwent the endovascular treatment with successfully retrieved clots from January 2017 to October 2020 were enrolled in the present study. Histological analysis was performed using hematoxylin and eosin (H&E) staining and immunostaining with CD3, CD20, CD105, and actin antibodies. CT obtained at the patients\' admission was to measure the attenuation and volume of all thrombus.
UNASSIGNED: A total of 94 subjects were included in this study. Fifty-six patients were classified as cardioembolic (CE), and 38 were classified with large-artery atherosclerosis (LAA). The subjects with LAA tend to exhibit higher actin and CD105 levels, and lower Hounsfield Unit (HU) values than subjects with CE. After adjusting for confounders, the actin was positively correlated with CD105 but not with HU values. Logistics regression shows actin was valuable for the prediction of LAA (OR, 1.148; 95% CI, 1.075-1.227; p < 0.001), even adjusted for age, sex, and intervention type (OR, 1.129; 95% CI, 1.048-1.216; p = 0.001), CT density and CD105 (OR, 1.161; 95% CI, 1.056-1.277; p = 0.002). Actin levels have a strong accuracy in differentiating LAA from CE, especially combined with CT density and CD105, which yielded a sensitivity of 63.2%, a specificity of 89.3%, with the area under the curve (AUC) at 0.821 (95% CI, 0.731-0.912).
UNASSIGNED: Our findings suggest that actin\'s level was a major factor differentiating atherothrombotic origin strokes from the cardioembolic stroke.
UNASSIGNED: ChiCTR2100051173.
UNASSIGNED: A total of 94 patients with AIS who underwent the endovascular treatment with successfully retrieved clots from January 2017 to October 2020 were enrolled in the present study. Histological analysis was performed using hematoxylin and eosin (H&E) staining and immunostaining with CD3, CD20, CD105, and actin antibodies. CT obtained at the patients\' admission was to measure the attenuation and volume of all thrombus.
UNASSIGNED: A total of 94 subjects were included in this study. Fifty-six patients were classified as cardioembolic (CE), and 38 were classified with large-artery atherosclerosis (LAA). The subjects with LAA tend to exhibit higher actin and CD105 levels, and lower Hounsfield Unit (HU) values than subjects with CE. After adjusting for confounders, the actin was positively correlated with CD105 but not with HU values. Logistics regression shows actin was valuable for the prediction of LAA (OR, 1.148; 95% CI, 1.075-1.227; p < 0.001), even adjusted for age, sex, and intervention type (OR, 1.129; 95% CI, 1.048-1.216; p = 0.001), CT density and CD105 (OR, 1.161; 95% CI, 1.056-1.277; p = 0.002). Actin levels have a strong accuracy in differentiating LAA from CE, especially combined with CT density and CD105, which yielded a sensitivity of 63.2%, a specificity of 89.3%, with the area under the curve (AUC) at 0.821 (95% CI, 0.731-0.912).
UNASSIGNED: Our findings suggest that actin\'s level was a major factor differentiating atherothrombotic origin strokes from the cardioembolic stroke.
UNASSIGNED: ChiCTR2100051173.
摘要：
■作为治疗目标，根据卒中亚型的不同，血栓的影像学信息和组织学特征可能有所不同。本研究旨在为脑卒中病因与CT非增强扫描的相关性研究提供依据。和急性缺血性中风（AIS）中检索到的凝块的组织学组成。
■从2017年1月至2020年10月，共有94例AIS患者接受了血管内治疗，并成功取回血凝块，纳入本研究。使用苏木精和伊红(H&E)染色以及用CD3、CD20、CD105和肌动蛋白抗体的免疫染色进行组织学分析。患者入院时获得的CT用于测量所有血栓的衰减和体积。
■本研究共纳入94名受试者。56例患者被归类为心脏栓塞（CE），38例被分类为大动脉粥样硬化（LAA）。LAA患者往往表现出更高的肌动蛋白和CD105水平，和低于CE受试者的Hounsfield单位（HU）值。在调整了混杂因素后，肌动蛋白与CD105呈正相关，但与HU值无关。物流回归显示肌动蛋白对LAA的预测有价值(OR，1.148；95%CI，1.075-1.227；p<0.001），甚至调整了年龄，性别,和干预类型(或，1.129；95%CI，1.048-1.216；p=0.001），CT密度和CD105(OR,1.161；95%CI，1.056-1.277；p=0.002）。肌动蛋白水平在区分LAA和CE方面具有很强的准确性，特别是结合CT密度和CD105，灵敏度为63.2%，特异性为89.3%，曲线下面积（AUC）为0.821（95%CI，0.731-0.912）。
■我们的研究结果表明，肌动蛋白水平是区分动脉粥样硬化血栓性卒中和心源性卒中的主要因素。
■ChiCTR2100051173。
■从2017年1月至2020年10月，共有94例AIS患者接受了血管内治疗，并成功取回血凝块，纳入本研究。使用苏木精和伊红(H&E)染色以及用CD3、CD20、CD105和肌动蛋白抗体的免疫染色进行组织学分析。患者入院时获得的CT用于测量所有血栓的衰减和体积。
■本研究共纳入94名受试者。56例患者被归类为心脏栓塞（CE），38例被分类为大动脉粥样硬化（LAA）。LAA患者往往表现出更高的肌动蛋白和CD105水平，和低于CE受试者的Hounsfield单位（HU）值。在调整了混杂因素后，肌动蛋白与CD105呈正相关，但与HU值无关。物流回归显示肌动蛋白对LAA的预测有价值(OR，1.148；95%CI，1.075-1.227；p<0.001），甚至调整了年龄，性别,和干预类型(或，1.129；95%CI，1.048-1.216；p=0.001），CT密度和CD105(OR,1.161；95%CI，1.056-1.277；p=0.002）。肌动蛋白水平在区分LAA和CE方面具有很强的准确性，特别是结合CT密度和CD105，灵敏度为63.2%，特异性为89.3%，曲线下面积（AUC）为0.821（95%CI，0.731-0.912）。
■我们的研究结果表明，肌动蛋白水平是区分动脉粥样硬化血栓性卒中和心源性卒中的主要因素。
■ChiCTR2100051173。
