Abstract：
The vascular endothelial cells constitute the innermost layer. The cells are exposed to mechanical stress by the flow, causing them to express their functions. To elucidate the functions, methods involving seeding endothelial cells as a layer in a chamber were studied. The chambers are known as parallel plate, T-chamber, step, cone plate, and stretch. The stimulated functions or signals from endothelial cells by flows are extensively connected to other outer layers of arteries or organs. The coculture layer was developed in a chamber to investigate the interaction between smooth muscle cells in the middle layer of the blood vessel wall in vascular physiology and pathology. Additionally, the microfabrication technology used to create a chamber for a microfluidic device involves both mechanical and chemical stimulation of cells to show their dynamics in in vivo microenvironments. The purpose of this study is to summarize the blood flow (flow inducing) for the functions connecting to endothelial cells and blood vessels, and to find directions for future chamber and device developments for further understanding and application of vascular functions. The relationship between chamber design flow, cell layers, and microfluidics was studied.
摘要：
血管内皮细胞构成最内层。细胞受到流动的机械应力，让他们表达自己的功能。为了阐明功能，研究了将内皮细胞作为小室中的一层进行接种的方法。这些腔室被称为平行板，T型室,步,锥板,和伸展。通过流动来自内皮细胞的刺激功能或信号广泛地连接到动脉或器官的其他外层。在腔室中开发共培养层，以研究血管生理学和病理学中血管壁中间层的平滑肌细胞之间的相互作用。此外,用于创建微流体装置的腔室的微加工技术涉及对细胞的机械和化学刺激，以显示其在体内微环境中的动力学。本研究的目的是总结与内皮细胞和血管连接的功能的血流（流动诱导），并为未来的腔室和设备发展寻找方向，以进一步理解和应用血管功能。腔室设计流程之间的关系，细胞层，研究了微流体。
