PDF(Pubmed)
Abstract：
Oxidative stress is the key factor that strengthens free radical generation which stimulates lung inflammation. The aim was to explore antioxidant, bronchodilatory along with anti-asthmatic potential of folkloric plants and the aqueous methanolic crude extract of Ipomoea nil (In.Cr) seeds which may demonstrate as more potent, economically affordable, having an improved antioxidant profile and providing evidence as exclusive therapeutic agents in respiratory pharmacology. In vitro antioxidant temperament was executed by DPPH, TFC, TPC and HPLC in addition to enzyme inhibition (cholinesterase) analysis; a bronchodilator assay on rabbit’s trachea as well as in vivo OVA-induced allergic asthmatic activity was performed on mice. In vitro analysis of 1,1-Diphenyl-2-picrylhydrazyl radical (DPPH) expressed as % inhibition 86.28 ± 0.25 with IC50 17.22 ± 0.56 mol/L, TPC 115.5 ± 1.02 mg GAE/g of dry sample, TFC 50.44 ± 1.06 mg QE/g dry weight of sample, inhibition in cholinesterase levels for acetyl and butyryl with IC50 (0.60 ± 0.67 and 1.5 ± 0.04 mol/L) in comparison with standard 0.06 ± 0.002 and 0.30 ± 0.003, respectively, while HPLC characterization of In.Cr confirmed the existence with identification as well as quantification of various polyphenolics and flavonoids i.e., gallic acid, vanillic acid, chlorogenic acid, quercetin, kaempferol and others. However, oral gavage of In.Cr at different doses in rabbits showed a better brochodilation profile as compared to carbachol and K+-induced bronchospasm. More significant (p < 0.01) reduction in OVA-induced allergic hyper-responses i.e., inflammatory cells grade, antibody IgE as well as altered IFN-α in airways were observed at three different doses of In.Cr. It can be concluded that sound mechanistic basis i.e., the existence of antioxidants: various phenolic and flavonoids, calcium antagonist(s) as well as enzymes’ inhibition profile, validates folkloric consumptions of this traditionally used plant to treat ailments of respiration.
摘要：
氧化应激是增强刺激肺部炎症的自由基生成的关键因素。目的是探索抗氧化剂，支气管扩张以及民间植物的抗哮喘潜力和罗汉果的甲醇水溶液粗提物（In。Cr)种子，可能表现出更强的效力，经济实惠,具有改善的抗氧化剂特性，并提供了作为呼吸药理学独家治疗剂的证据。体外抗氧化气质由DPPH执行，TFC,除了酶抑制（胆碱酯酶）分析外，还有TPC和HPLC；对小鼠进行了兔气管支气管扩张剂测定以及体内OVA诱导的过敏性哮喘活性。1,1-二苯基-2-吡啶酰肼基（DPPH）的体外分析表示为抑制％86.28±0.25，IC50为17.22±0.56mol/L，TPC115.5±1.02mgGAE/g干样品，TFC50.44±1.06mgQE/g样品干重，与标准的0.06±0.002和0.30±0.003相比，乙酰和丁酰基的胆碱酯酶水平分别具有IC50（0.60±0.67和1.5±0.04mol/L），而In的HPLC表征。Cr通过鉴定和定量确认了各种多酚和类黄酮的存在，即没食子酸,香草酸,绿原酸,槲皮素,山奈酚和其他人。然而,口服灌胃。与卡巴胆碱和K引起的支气管痉挛相比，不同剂量的Cr在兔子中显示出更好的溴扩张曲线。OVA诱导的过敏性过度反应更显著(p<0.01)减少，即炎症细胞等级，在三种不同剂量的In下观察到气道中的抗体IgE以及改变的IFN-α。Cr.可以得出结论，健全的机械基础，即，抗氧化剂的存在：各种酚类和类黄酮，钙拮抗剂以及酶的抑制谱，验证了这种传统植物的民俗消费，以治疗呼吸疾病。
