Five New Polyoxypregnane Glycosides from the Vines of Aspidopterysobcordata and Their Antinephrolithiasis Activity.

五株新的多羟基孕烷糖苷及其抗肾结石活性。

• 影响指数: 4.927
• 发表时间: Jul 2022 19
• 来源期刊: Molecules
• DOI: 10.3390/molecules27144596
• PMID: 35889467
• 文章类型: Journal Article
• 中科院分区: Q3
• 作者列表: Sun Z,Chen M,Li Q,Ma G,Wu H,Yang J,Li Y,Xu X,Sun Z,Chen M,Li Q,Ma G,Wu H,Yang J,Li Y,Xu X,Sun Z,Chen M,Li Q,Ma G,Wu H,Yang J,Li Y,Xu X

关键词: Aspidopterys obcordata; HK-2 cells; antinephrolithiasis; polyoxypregnane glycosides; Aspidopterys obcordata; HK-2 cells; antinephrolithiasis; polyoxypregnane glycosides; Aspidopterys obcordata; HK-2 cells; antinephrolithiasis; polyoxypregnane glycosides

Mesh: Drugs, Chinese Herbal / chemistry; Glycosides / chemistry pharmacology; Humans; Malpighiaceae / chemistry; Molecular Structure; Saponins / chemistry; Drugs, Chinese Herbal / chemistry; Glycosides / chemistry pharmacology; Humans; Malpighiaceae / chemistry; Molecular Structure; Saponins / chemistry

Abstract

From the dried vines of Aspidopterys obcordata Hemsl, five new polyoxypregnane glycosides, named obcordatas J-N (1-5), were obtained. Their structures were fully elucidated and characterized by HRESIMS and extensive spectroscopic data. In addition, all of the new compounds were screened for their antinephrolithiasis activity in vitro. The results showed that compounds 1-3 have prominent protective effects on calcium oxalate crystal-induced human kidney 2 (HK-2) cells, with EC50 values ranging from 6.72 to 14.00 μM, which is consistent with the application value of A. obcordata in folk medicine for kidney stones.

摘要

从AspidopterysobcordataHemsl的干燥藤本植物中，五种新的聚氧孕烷苷，命名为obcordatasJ-N（1-5），已获得。通过HRESIMS和广泛的光谱数据充分阐明和表征了它们的结构。此外,所有新化合物均在体外筛选出抗肾结石活性。结果表明,化合物1-3对草酸钙晶体诱导的人肾2(HK-2)细胞具有显著的保护作用,EC50值范围为6.72至14.00μM，这与A.obcordata在民间治疗肾结石医学中的应用价值是一致的。