Abstract：
(1) Background: Vaccination of dogs against leptospirosis is of paramount importance, as they ideally must provide not only long-term protection, but also against the renal carrier state of leptospires. This study assessed the post-vaccine humoral response against Leptospira in naturally exposed dogs and effects on renal carrier status. (2) Methods: A total of 118 dogs were studied for 365 days, separated into Group A (vaccinated, n = 94) and Group B (non-vaccinated, n = 24). Group A was subdivided into three groups: A1 with 32 dogs immunized with the vaccine #1; A2 by 32 dogs with #2; and A3 30 dogs with #3. Serology (MAT and IgG-ELISA) and urinary PCR were conducted. (3) Results: Seroreactivity increased at D15 post-vaccination and, regardless of vaccine brand, remained high up to D180, with antibody switch to IgG after D30. A total of 46.8% of animals from Group A were PCR-positive at least once, in contrast to 75% in Group B, regardless of vaccine brand (p < 0.05; OR: 0.3). (4) Conclusions: All commercial vaccines succeeded at eliciting a long-term IgG-based response and were partially effective at protecting against kidney infection.
摘要：
(1)背景:狗接种钩端螺旋体病疫苗至关重要,理想情况下，它们不仅必须提供长期保护，而且还对抗肾携带钩端螺旋体的状态。这项研究评估了自然暴露的狗中针对钩端螺旋体的疫苗后体液反应以及对肾脏携带者状态的影响。(2)方法:对118只犬进行了365天的研究,分为A组(接种疫苗，n=94）和B组（未接种疫苗，n=24）。将A组细分为三组:A1,用疫苗#1免疫32只狗;A2,用#2免疫32只狗;和A3,用#3狗。进行血清学（MAT和IgG-ELISA）和尿液PCR。（3）结果：疫苗接种后D15血清反应性增加，无论疫苗品牌，保持高至D180，D30后抗体转换为IgG。来自A组的46.8％的动物至少一次PCR阳性，与B组的75%相比，无论疫苗品牌（p<0.05；OR：0.3）。(4)结论:所有商业疫苗均成功地引发基于IgG的长期应答,并且部分有效地防止肾脏感染。
