Abstract：
Human milk oligosaccharides (HMOs) have attracted increasing attention due to the emerging evidence of their positive roles for infant\'s health. A high-throughput method for absolute quantitation of the complex HMOs including multiple isomeric structures is important but very challenging, due to the highly divers nature and wide variation in content of HMOs from different individuals. Here we used UPLC-MS-MRM in the negative-ion mode for accurate quantitation of 23 complex HMOs in just 15 min. The selected oligosaccharides are in their native forms and include neutral and sialylated, fucosylated and non-fucosylated, linear and branched, and secretor and Lewis phenotype indicators. The well validated method with good sensitivity, recovery and reproducibility was then applied to a large population quantitative survey of 251 Chinese mothers from five different ethnic groups (Han, Zhuang, Hui, Mongolian and Tibetan) living in different geographical regions for their secretor\'s status and Lewis phenotypes.
摘要：
人乳低聚糖(HMO)因其对婴儿健康的积极作用而受到越来越多的关注。对包括多个异构结构的复杂HMO进行绝对定量的高通量方法很重要，但非常具有挑战性。由于高度潜水员的性质和来自不同个体的HMO含量差异很大。在这里，我们在负离子模式下使用UPLC-MS-MRM在短短15分钟内准确定量23个复杂的HMO。所选择的寡糖是其天然形式，包括中性和唾液酸化，岩藻糖基化和非岩藻糖基化，直链和支链，以及分泌者和刘易斯表型指标。该方法经过充分验证，具有良好的灵敏度，然后将恢复和再现性应用于来自五个不同种族的251名中国母亲的大人群定量调查(汉族，庄,Hui,蒙古族和藏族）因其分泌者的身份和刘易斯表型而生活在不同的地理区域。
