PDF(Pubmed)
Abstract：
UNASSIGNED: Leaves of Urtica simensis (U. simensis) have been used traditionally for wound healing in different communities in Ethiopia. In spite of this, there were no scientific data documented regarding the wound healing activity of this plant. There is a need to investigate herbal remedies for the treatment of wounds in order to overcome the limitations of conventional drugs.
UNASSIGNED: Aim of the study was to evaluate the wound healing activity of extract and solvent fractions of the leaves of U. simensis in mice.
UNASSIGNED: Leaves of U. simensis were washed, dried under shade and ground into coarse powder and then extracted by 80% methanol with three consecutive macerations. Part of the extract was fractionated with n-hexane, chloroform and water. In excision and burn wounds, healing progress was measured by wound contraction, epithelialization period and histopathology investigation whereas incision wound healing was assessed by skin breaking strength.
UNASSIGNED: In excision wound model, the 5% and 10% crude extract ointments showed significant (p < 0.001) wound contractions during day 8 to day 16 evaluations. Similarly, in burn wound model, both 5% and 10% crude extract ointments produced significant (p < 0.001) wound contractions starting from day 12 and 10, respectively. In both models, the periods of epithelialization were also significantly reduced and favorable histopathologic changes were produced by the crude extract ointments. The solvent fractions of the crude extract as well produced significant wound contractions as evaluated in excision wound model. The fractions also significantly reduced the period of epithelialization in this model. The aqueous fraction found to be more active than either chloroform or n-hexane fraction in wound healing.
UNASSIGNED: Results of this study indicated that methanol extract and aqueous fractions of the leaves of U. simensis possess dose-dependent wound healing activity, thus supporting traditional claims.
UNASSIGNED: Aim of the study was to evaluate the wound healing activity of extract and solvent fractions of the leaves of U. simensis in mice.
UNASSIGNED: Leaves of U. simensis were washed, dried under shade and ground into coarse powder and then extracted by 80% methanol with three consecutive macerations. Part of the extract was fractionated with n-hexane, chloroform and water. In excision and burn wounds, healing progress was measured by wound contraction, epithelialization period and histopathology investigation whereas incision wound healing was assessed by skin breaking strength.
UNASSIGNED: In excision wound model, the 5% and 10% crude extract ointments showed significant (p < 0.001) wound contractions during day 8 to day 16 evaluations. Similarly, in burn wound model, both 5% and 10% crude extract ointments produced significant (p < 0.001) wound contractions starting from day 12 and 10, respectively. In both models, the periods of epithelialization were also significantly reduced and favorable histopathologic changes were produced by the crude extract ointments. The solvent fractions of the crude extract as well produced significant wound contractions as evaluated in excision wound model. The fractions also significantly reduced the period of epithelialization in this model. The aqueous fraction found to be more active than either chloroform or n-hexane fraction in wound healing.
UNASSIGNED: Results of this study indicated that methanol extract and aqueous fractions of the leaves of U. simensis possess dose-dependent wound healing activity, thus supporting traditional claims.
摘要：
■小熊叶（U.simensis）传统上已在埃塞俄比亚的不同社区中用于伤口愈合。尽管如此,没有关于该植物伤口愈合活性的科学数据记录。需要研究用于治疗伤口的草药以克服常规药物的局限性。
■本研究的目的是评估山葵叶的提取物和溶剂级分在小鼠中的伤口愈合活性。
■洗了山豆叶，在阴凉处干燥，磨成粗粉末，然后用80%甲醇提取，连续三次浸渍。部分提取物用正己烷分馏，氯仿和水。在切除和烧伤伤口中，愈合进展是通过伤口收缩来衡量的，上皮形成期和组织病理学研究，而切口伤口愈合通过皮肤断裂强度进行评估。
■在切除伤口模型中，在第8天至第16天的评价期间,5%和10%粗提取物软膏显示出显著(p<0.001)的伤口收缩。同样，在烧伤伤口模型中，5%和10%粗提取物软膏分别从第12天和第10天开始产生显著(p<0.001)的伤口收缩。在这两种模型中，上皮形成的时间也显著缩短,粗提取物软膏产生了良好的组织病理学变化.粗提取物的溶剂级分也产生显著的伤口收缩,如在切除伤口模型中评估的。在该模型中,这些部分还显著减少了上皮形成的周期。发现水性部分在伤口愈合中比氯仿或正己烷部分更具活性。
■这项研究的结果表明，山梨叶的甲醇提取物和水性部分具有剂量依赖性的伤口愈合活性，从而支持传统主张。
■本研究的目的是评估山葵叶的提取物和溶剂级分在小鼠中的伤口愈合活性。
■洗了山豆叶，在阴凉处干燥，磨成粗粉末，然后用80%甲醇提取，连续三次浸渍。部分提取物用正己烷分馏，氯仿和水。在切除和烧伤伤口中，愈合进展是通过伤口收缩来衡量的，上皮形成期和组织病理学研究，而切口伤口愈合通过皮肤断裂强度进行评估。
■在切除伤口模型中，在第8天至第16天的评价期间,5%和10%粗提取物软膏显示出显著(p<0.001)的伤口收缩。同样，在烧伤伤口模型中，5%和10%粗提取物软膏分别从第12天和第10天开始产生显著(p<0.001)的伤口收缩。在这两种模型中，上皮形成的时间也显著缩短,粗提取物软膏产生了良好的组织病理学变化.粗提取物的溶剂级分也产生显著的伤口收缩,如在切除伤口模型中评估的。在该模型中,这些部分还显著减少了上皮形成的周期。发现水性部分在伤口愈合中比氯仿或正己烷部分更具活性。
■这项研究的结果表明，山梨叶的甲醇提取物和水性部分具有剂量依赖性的伤口愈合活性，从而支持传统主张。
