Abstract：
Tissue-engineered heart valves (TEHVs) are the most promising replacement for heart valve transplantation. Decellularized heart valve (DHV) is one of the most common scaffold materials for TEHVs. In actual clinical applications, the most widely used method for treating DHV is cross-linking it with glutaraldehyde, but this method could cause serious problems such as calcification. In this study, we introduced polyhedral oligomeric silsesquioxane (POSS) nanoparticles into a poly(ethylene glycol) (PEG) hydrogel to prepare a POSS-PEG hybrid hydrogel, and then coated them on the surface of DHV to prepare the composite scaffold. The chemical structures, microscopic morphologies, cell compatibilities, blood compatibilities, and anticalcification properties were further investigated. Experimental results showed that the composite scaffold had good blood compatibility and excellent cell compatibility and could promote cell adhesion and proliferation. In vivo and in vitro anticalcification experiments showed that the introduction of POSS nanoparticles could reduce the degree of calcification significantly and the composite scaffold had obvious anticalcification ability. The DHV surface-coated with the POSS-PEG hybrid hydrogel is an alternative scaffold material with anticalcification potential for an artificial heart valve, which provides an idea for the preparation of TEHVs.
摘要：
组织工程心脏瓣膜(TEHVs)是最有希望的心脏瓣膜移植替代物。脱细胞心脏瓣膜(DHV)是最常用的TEHV支架材料之一。在实际的临床应用中,处理DHV最广泛使用的方法是用戊二醛交联，但是这种方法可能会导致严重的问题，如钙化。在这项研究中,我们将多面体低聚倍半硅氧烷(POSS)纳米颗粒引入到聚乙二醇(PEG)水凝胶中,然后将它们涂覆在DHV的表面上制备复合支架。化学结构，微观形态,细胞相容性，血液相容性,和抗钙化性能进行了进一步研究。实验结果表明，复合支架具有良好的血液相容性和良好的细胞相容性，能促进细胞的黏附和增殖。体内和体外抗钙化实验表明，POSS纳米颗粒的引入能显著降低钙化程度，复合支架具有明显的抗钙化能力。表面涂覆有POSS-PEG混合水凝胶的DHV是一种具有抗钙化潜力的人造心脏瓣膜的替代支架材料。这为制备TEHVs提供了思路。
