Abstract：
Measuring myocardial contractility is of great value in exploring cardiac pathogenesis and quantifying drug efficacy. Among the biosensing platforms developed for detecting the weak contractility of a single layer of cardiomyocytes (CMs), thin brittle metal membrane sensors with microcracks are highly sensitive. However, their poor stability limits the application in long-term measurement. Here, we report a high stability crack sensor fabricated by deposition of a 105 nm thick Ag/Cr with microcracks onto a carbon nanotubes-polydimethylsiloxane (CNT-PDMS) layer. This brittle-tough bilayer crack sensor achieved high sensitivity (gauge factor: 108 241.7), a wide working range (0.01-44%), and high stability (stable period >2 000 000 cycles under the strain caused by a monolayer of CMs). During 14-day continuously monitoring CMs culturing and drug treatment testings, the device demonstrated high sensitivity and stability to record the dynamic change caused by contractility of the CMs.
摘要：
测量心肌收缩力在探索心脏发病机制和量化药物疗效方面具有重要价值。在为检测单层心肌细胞（CM）的弱收缩性而开发的生物传感平台中，具有微裂纹的薄脆性金属膜传感器高度敏感。然而,稳定性差限制了其在长期测量中的应用。这里,我们报告了一种高稳定性的裂纹传感器，该传感器通过在碳纳米管-聚二甲基硅氧烷（CNT-PDMS）层上沉积具有微裂纹的105nm厚的Ag/Cr来制造。这种脆性坚韧的双层裂纹传感器实现了高灵敏度（规格系数：108241.7），宽工作范围(0.01-44%)，和高稳定性(在单层CM引起的应变下，稳定期>2000000个循环)。在为期14天的连续监测CMs培养和药物治疗测试中，
