Abstract:
Fat deposition is an important economic trait that is closely related to feed efficiency and carcass performance in livestock. In this study, the fat deposition-related traits of 1,293 Hu sheep were measured and descriptive statistical analysis was conducted. The results showed that the coefficient of variation of all fat deposition-related traits was higher than 24%. In addition, single nucleotide polymorphisms and the expression characteristics of TRAPPC9 (encoding trafficking protein particle complex subunit 9) and BAIAP2 (encoding brain-specific Angiogenesis inhibitor 1-associated protein 2) genes in Hu sheep were detected using PCR amplification, Sanger sequencing, KASPar genotyping, and quantitative real-time reverse transcription PCR (qRT-PCR). The associations between SNPs and fat deposition-related traits were also analyzed. Two intronic mutations, TRAPPC9 g.57654 A > G and BAIAP2 g.46061 C > T, were identified in Hu sheep. The result of association analysis showed that TRAPPC9 g.57654 A > G and BAIAP2 g.46061 C > T were both significantly associated with the weight of tail fat, tail fat relative weight (body weight), and tail fat relative weight (carcass) (P < 0.05). Comprehensive effects analysis showed that there were significant differences between the combined genotypes and tail fat and perirenal fat deposition. Moreover, qRT-PCR analysis showed that TRAPPC9 and BAIAP2 are widely expressed, and their expression levels were significantly higher in the small-tail group compared with those in the big-tail group (P < 0.01). These results provided important candidate molecular markers that could be used in strategies to reduce tail fat deposition in Hu sheep.
摘要:
脂肪沉积是重要的经济性状,与牲畜的饲料效率和car体性能密切相关。在这项研究中,对1293只湖羊的脂肪沉积相关性状进行了测定和描述性统计分析。结果表明,所有脂肪沉积相关性状的变异系数均高于24%。此外,用PCR扩增法检测湖羊TRAPPC9(编码运输蛋白颗粒复合体亚基9)和BAIAP2(编码脑特异性血管生成抑制因子1相关蛋白2)基因的单核苷酸多态性和表达特征,桑格测序,KASPar基因分型,和定量实时逆转录PCR(qRT-PCR)。还分析了SNP与脂肪沉积相关性状之间的关联。两个内含子突变,TRAPPC9g.57654A>G和BAIAP2g.46061C>T,在胡羊身上被发现。关联分析结果表明,TRAPPC9g.57654A>G和BAIAP2g.46061C>T均与尾脂重量显著相关,尾巴脂肪相对重量(体重),和尾脂肪相对重量(胴体)(P<0.05)。综合效应分析表明,组合基因型与尾脂和肾周脂肪沉积之间存在显著差异。此外,qRT-PCR分析显示TRAPPC9和BAIAP2广泛表达,小尾组的表达水平明显高于大尾组(P<0.01)。这些结果提供了重要的候选分子标记,可用于减少湖羊尾部脂肪沉积的策略。