Abstract:
Photosynthetic microorganisms, specifically cyanobacteria and microalgae, can synthesize a vast array of biologically active molecules, such as lectins, that have great potential for various biotechnological and biomedical applications. However, since the structures of these proteins are not well established, likely due to the presence of intrinsically disordered regions, our ability to better understand their functionality is hampered. We embarked on a study of the carbohydrate recognition domain (CRD), intrinsically disordered regions (IDRs), amino acidic composition, as well as and functional motifs in lectins from cyanobacteria of the genus Arthrospira and microalgae Chlorella and Dunaliella genus using a combination of bioinformatics techniques. This search revealed the presence of five distinctive CRD types differently distributed between the genera. Most CRDs displayed a group-specific distribution, except to C. sorokiniana possessing distinctive CRD probably due to its specific lifestyle. We also found that all CRDs contain short IDRs. Bacterial lectin of Arthrospira prokarionte showed lower intrinsic disorder and proline content when compared to the lectins from the eukaryotic microalgae (Chlorella and Dunaliella). Among the important functions predicted in all lectins were several specific motifs, which directly interacts with proteins involved in the cell-cycle control and which may be used for pharmaceutical purposes. Since the aforementioned properties of each type of lectin were investigated in silico, they need experimental confirmation. The results of our study provide an overview of the distribution of CRD, IDRs, and functional motifs within lectin from the commercially important microalgae.
摘要:
光合微生物,特别是蓝藻和微藻,可以合成大量的生物活性分子,如凝集素,在各种生物技术和生物医学应用中具有巨大潜力。然而,因为这些蛋白质的结构还没有很好的建立,可能是由于内在无序区域的存在,我们更好地理解它们的功能的能力受到了阻碍。我们开始了碳水化合物识别域(CRD)的研究,内在无序区域(IDR),氨基酸组成,以及使用生物信息学技术相结合的节旋藻属蓝藻和微藻小球藻和杜氏藻属的凝集素中的功能基序。此搜索显示存在五种不同的CRD类型,它们在属之间分布不同。大多数CRD显示特定于组的分布,除了C.sorokiniana具有独特的CRD可能是由于其特定的生活方式。我们还发现所有CRD都包含短IDR。与真核微藻(小球藻和杜氏藻)的凝集素相比,Prokarionte的细菌凝集素显示出较低的内在紊乱和脯氨酸含量。在所有凝集素中预测的重要功能中有几个特定的基序,直接与参与细胞周期控制的蛋白质相互作用,可用于药物目的。由于每种凝集素的上述性质都是在计算机上进行研究的,他们需要实验确认。我们的研究结果提供了CRD分布的概述,IDR,和来自商业上重要的微藻的凝集素中的功能基序。