Abstract：
Despite the worldwide use of 16S rRNA to identify bacterial species, the use of this gene does not discriminate the 750 species in the genus Streptomyces. A MLST scheme was constructed with rpoB, gyrB, recA, trpB and atpD genes to access the genomic variances in Streptomyces species evolution. We analyze the housekeeping genes in 49 Streptomyces isolates from Antarctic soil. It was used two different databases, GenBank and EzBioCloud to compare the 16S sequences. The species founded in both databases are not the same, but in both cases, a few isolates achieve the necessary high percentage to consider the identification. There is a lack of deposited sequences in the other genes, as the data in GenBank proved to be insufficient. Isolate LMA323St_9 has the potential to be studied as a novel species. Besides that, the use of housekeeping genes gives robust phylogenetic information to understand in group relationships.
摘要：
尽管全世界都在使用16SrRNA来鉴定细菌种类，该基因的使用不会区分链霉菌属中的750种。使用rpoB构建了MLST方案，gyrB,recA，trpB和atpD基因访问链霉菌物种进化中的基因组变异。我们分析了来自南极土壤的49种链霉菌中的管家基因。它使用了两个不同的数据库，GenBank和EzBioCloud比较16S序列。两个数据库中建立的物种并不相同，但在这两种情况下，少数分离株达到必要的高百分比以考虑鉴定。其他基因缺乏保藏序列，因为GenBank中的数据被证明是不够的。分离物LMA323St_9具有作为新物种进行研究的潜力。除此之外,管家基因的使用提供了强大的系统发育信息来理解群体关系。
