Abstract：
It has been well investigated that the bioluminescence (BL) intensity of marine luminous bacteria is enhanced depending on cell density. In contrast, the correlation between seawater components and BL intensity is still a challenging subject to be addressed. In addition, the marine luminous bacteria rapidly lose the BL intensity when exposed to toxic substances, but unclear to fungicides. Herein, we introduce a new approach to investigate (i) the correlation between the components of artificial seawater (ASW) and BL intensity and (ii) the corresponding protocol to determine the susceptibility of marine luminous bacteria to fungicide using A. fischeri. The examples show that (i) ionic ingredients (K+, HCO3-, and SO42-) activate the BL cell density independently and (ii) A. fischeri cultured with the ionic ingredients shows the susceptibility to fungicide (sodium ortho-phenylphenol and imazalil). These protocols provide a new insight how to investigate the correlation between inorganic salts and BL intensity in a low cell density environment such as seawater.
摘要：
已经很好地研究了海洋发光细菌的生物发光（BL）强度根据细胞密度而增强。相比之下,海水成分与BL强度之间的相关性仍然是一个具有挑战性的课题。此外,海洋发光细菌在暴露于有毒物质时迅速失去BL强度，但对杀菌剂不清楚。在这里，我们引入了一种新的方法来研究（i）人工海水（ASW）成分与BL强度之间的相关性，以及（ii）使用Fischeri确定海洋发光细菌对杀菌剂的敏感性的相应方案。实施例表明，(i)离子成分(K+，HCO3-,和SO42-）独立地激活BL细胞密度，并且（ii）与离子成分一起培养的Fischeri显示出对杀菌剂（邻苯基苯酚钠和imazalil）的敏感性。这些方案提供了如何在低细胞密度环境(如海水)中研究无机盐与BL强度之间的相关性的新见解。
