Abstract：
OBJECTIVE: Chromodomain helicase DNA-binding 5 (CHD5) acts as a tumor suppressor gene in some cancers. CHD5 expression levels may affect an individual\'s susceptibility to hepatocellular carcinoma (HCC). This study aimed to evaluate the methylation pattern of the CHD5 promoter region and the gene\'s corresponding mRNA expression in HCC patients compared with healthy individuals.
METHODS: In this case-control study, CHD5 mRNA gene expression levels and DNA methylation patterns were analyzed in 81 HCC patients and 90 healthy individuals by quantitative reverse transcription polymerase chain reaction and methylation-specific polymerase chain reaction, respectively.
RESULTS: The CHD5 gene was hypermethylated in 61.8% of the HCC patients and 54.4% of the controls, and this difference was statistically significant. The CHD5 mRNA expression levels were significantly lower in the HCC patient group.
CONCLUSIONS: Hypermethylation of the CHD5 promoter region may significantly lower the expression of this gene, affecting the incidence and severity of HCC. The methylation status of CHD5 can also be further studied as a prognostic factor in HCC.
METHODS: In this case-control study, CHD5 mRNA gene expression levels and DNA methylation patterns were analyzed in 81 HCC patients and 90 healthy individuals by quantitative reverse transcription polymerase chain reaction and methylation-specific polymerase chain reaction, respectively.
RESULTS: The CHD5 gene was hypermethylated in 61.8% of the HCC patients and 54.4% of the controls, and this difference was statistically significant. The CHD5 mRNA expression levels were significantly lower in the HCC patient group.
CONCLUSIONS: Hypermethylation of the CHD5 promoter region may significantly lower the expression of this gene, affecting the incidence and severity of HCC. The methylation status of CHD5 can also be further studied as a prognostic factor in HCC.
摘要：
目的：染色体域解旋酶DNA结合5（CHD5）在某些癌症中充当抑癌基因。CHD5表达水平可能影响个体对肝细胞癌(HCC)的易感性。这项研究旨在评估CHD5启动子区的甲基化模式和基因的相应mRNA表达在肝癌患者与健康个体相比。
方法：在本病例对照研究中，通过定量逆转录聚合酶链反应和甲基化特异性聚合酶链反应分析81例HCC患者和90例健康个体的CHD5mRNA基因表达水平和DNA甲基化模式。分别。
结果：CHD5基因在61.8%的HCC患者和54.4%的对照组中高度甲基化，这种差异具有统计学意义。CHD5mRNA表达水平在HCC患者组中显著降低。
结论：CHD5启动子区的超甲基化可能显著降低该基因的表达，影响HCC的发病率和严重程度。CHD5的甲基化状态也可以作为HCC的预后因素进一步研究。
方法：在本病例对照研究中，通过定量逆转录聚合酶链反应和甲基化特异性聚合酶链反应分析81例HCC患者和90例健康个体的CHD5mRNA基因表达水平和DNA甲基化模式。分别。
结果：CHD5基因在61.8%的HCC患者和54.4%的对照组中高度甲基化，这种差异具有统计学意义。CHD5mRNA表达水平在HCC患者组中显著降低。
结论：CHD5启动子区的超甲基化可能显著降低该基因的表达，影响HCC的发病率和严重程度。CHD5的甲基化状态也可以作为HCC的预后因素进一步研究。
