Abstract：
Since ancient times, medicinal plants are widely accepted to promote the health and wellness of animals and mankind. The medicinal plant-based therapies have limitations of delayed onset of action, inconsistent absorption, low bioavailability, oxidation, and poor solubility. The encapsulation studies suggested improved efficacy. Therefore, the present study attempts to evaluate the efficacy of Curcuma longa extracts encapsulated in Ethosome on wound healing model compared to crude extract. The Curcuma longa extract swere prepared by cold percolation method and total curcuminoid content was determined by Reverse phase-HPLC. Three Ethosomal suspensions (ETS1, ETS2, and ETS3) were prepared and characterized for particle distribution, morphology, and absorption spectrum by Zetasizer, Scanning Electron Microscopy, and FTIR respectively. The Ethosomal suspension with the highest entrapment efficiency was applied topically at a varying concentrations (0.25, 0.5, and 1 g/cm2) on the surgically created wounds in rats. The efficacy of wound healing was evaluated by clinical observation, macroscopic evaluation of granulation tissue, colour digital image processing, and histology. The methanolic extract of Curcuma longa showed better antibacterial potential than ethanolic and aqueous. The total Curcuminoid content in the Curcuma longa rhizome was 4.03%. The size, PDI, zeta potential, and viscosity of Ethosomal suspension ranged from 34.8 to 371 nm, 0.236-1.178, 15.6-36.8mV, and 0.8460-0.8510, respectively. The ETS3 was found the most optimum combination with the highest entrapment efficiency and the topical application at a dose rate of 0.5 g/cm2 and 1.0 g/cm2 resulted in comparable wound contracture, pain score, histopathological score as compared to control groups.It was concluded that the Curcuma longa encapsulation in Ethosome resulted in improved wound appearance, granulation tissue score, and appearance with a shortened period of wound resolution at the cellular level as compared to crude extract.
摘要：
自古以来,药用植物被广泛接受，以促进动物和人类的健康和健康。药用植物疗法具有延迟起效的局限性，不一致的吸收，低生物利用度，氧化，溶解性差。包封研究表明功效改善。因此,本研究试图评估Ethosome封装的姜黄提取物与粗提取物相比对伤口愈合模型的功效。采用冷渗滤法制备姜黄提取物，并采用反相高效液相色谱法测定总姜黄素含量。制备了三种乙醇混悬液（ETS1，ETS2和ETS3），并对其颗粒分布进行了表征。形态学,和Zetasizer的吸收光谱，扫描电子显微镜,和FTIR分别。将具有最高包封效率的乙醇体混悬液以不同浓度(0.25、0.5和lg/cm2)局部施用于大鼠手术产生的伤口上。通过临床观察评价创面愈合的疗效,肉芽组织的宏观评价，彩色数字图像处理，和组织学。姜黄的甲醇提取物显示出比乙醇和水溶液更好的抗菌潜力。姜黄根茎中总姜黄素含量为4.03%。大小,PDI,zeta电位,eyosomal悬浮液的粘度范围为34.8至371nm，0.236-1.178,15.6-36.8mV,分别为0.8460-0.8510。发现ETS3是具有最高包封效率的最佳组合，并且在0.5g/cm2和1.0g/cm2的剂量率下局部应用导致了相当的伤口挛缩。疼痛评分,与对照组相比，组织病理学评分。结论是，姜黄在Ethosome中的封装导致改善的伤口外观，肉芽组织评分,与粗提物相比，在细胞水平上的伤口消退时间缩短。
