Abstract：
In this paper, the S-nitrosylated myofibrillar protein in pork was comparatively analyzed between pale, soft, and exudative (PSE) and red, firm, and non-exudative (RFN) meat. The S-nitrosothiol and immunoblot of S-nitrosylated protein indicated that the overall protein S-nitrosylation level in PSE pork was higher than that in RFN pork. Proteomics showed that 114 SNO-modified cysteines corresponding to 65 proteins were over-expressed in PSE samples while 74 nitrosylated cysteines of 20 proteins were over-expressed in RFN samples. Differential proteins including myosin, actin, actinin, nebulin, titin, troponin-I, and filamin were distributed in the cytoskeleton and muscle fibers, participating in muscle contraction, cell development, and myofibril assembly by exerting binding activity. The enriched KEGG pathways included tight junction, regulation of actin cytoskeleton, glycolysis/gluconeogenesis, AMPK signaling pathway, and HIF-1 signaling pathway. Our data suggest that S-nitrosylation of myofibrillar protein could be an alternative pathway of NO involved in the regulation of fresh meat quality.
摘要：
在本文中,比较分析了猪肉中的S-亚硝基肌原纤维蛋白，软,渗出性（PSE）和红色，公司，和非渗出性（RFN）肉。S-亚硝基硫醇和S-亚硝基化蛋白的免疫印迹表明，PSE猪肉中的整体蛋白S-亚硝基化水平高于RFN猪肉。蛋白质组学显示,对应于65种蛋白质的114个SNO修饰的半胱氨酸在PSE样品中过表达,而20种蛋白质的74个亚硝基化的半胱氨酸在RFN样品中过表达。差异蛋白包括肌球蛋白,肌动蛋白，actinin,星云,替丁,肌钙蛋白-I,丝素分布在细胞骨架和肌纤维中，参与肌肉收缩，细胞发育,和肌原纤维组装通过发挥结合活性。丰富的KEGG途径包括紧密连接，肌动蛋白细胞骨架的调节，糖酵解/糖异生，AMPK信号通路,和HIF-1信号通路。我们的数据表明，肌原纤维蛋白的S-亚硝基化可能是NO参与新鲜肉品质调节的替代途径。
