PDF(Pubmed)
Abstract：
Early flowering, maturity, and plant height are important traits for linseed to fit in rice fallows, for rainfed agriculture, and for economically viable cultivation. Here, Multi-Locus Genome-Wide Association Study (ML-GWAS) was undertaken in an association mapping panel of 131 accessions, genotyped using 68,925 SNPs identified by genotyping by sequencing approach. Phenotypic evaluation data of five environments comprising 3 years and two locations were used. GWAS was performed for three flowering time traits including days to 5%, 50%, and 95% flowering, days to maturity, and plant height by employing five ML-GWAS methods: FASTmrEMMA, FASTmrMLM, ISIS EM-BLASSO, mrMLM, and pLARmEB. A total of 335 unique QTNs have been identified for five traits across five environments. 109 QTNs were stable as observed in ≥2 methods and/or environments, explaining up to 36.6% phenotypic variance. For three flowering time traits, days to maturity, and plant height, 53, 30, and 27 stable QTNs, respectively, were identified. Candidate genes having roles in flower, pollen, embryo, seed and fruit development, and xylem/phloem histogenesis have been identified. Gene expression of candidate genes for flowering and plant height were studied using transcriptome of an early maturing variety Sharda (IC0523807). The present study unravels QTNs/candidate genes underlying complex flowering, days to maturity, and plant height traits in linseed.
摘要：
早期开花，成熟，和株高是亚麻籽适合水稻休耕的重要性状，对于雨养农业，为了经济上可行的种植。这里,多基因座全基因组关联研究（ML-GWAS）在131个种质的关联映射面板中进行，使用通过测序方法进行基因分型鉴定的68,925个SNP进行基因分型。使用包括3年和两个地点的五个环境的表型评估数据。GWAS进行了三个开花时间性状，包括天数到5%，50%,95%开花，天成熟,通过采用五种ML-GWAS方法：FASTmrEMMA，FASTmrMLM,ISISEM-BLASSO,mrMLM,和pLARmEB。在五个环境中，共有335个独特的QTNs被鉴定为五个性状。109个QTNs在≥2种方法和/或环境中观察到稳定，解释高达36.6%的表型变异。对于三个开花时间性状，天成熟,和植物高度，53、30和27个稳定的QTNs，分别,已确定。在花中发挥作用的候选基因，花粉,胚胎,种子和果实发育，木质部/韧皮部组织发生已经确定。使用早熟品种Sharda（IC0523807）的转录组研究了开花和植物高度候选基因的基因表达。本研究揭示了复杂开花的QTNs/候选基因，天成熟,亚麻籽的株高性状。
