Abstract：
Iron tailings matrix is deficient in nutrients, and phytoremediation is one of the effective methods to improve tailings nutrients. The response of phytoremediation to tailings microorganisms remains to be studied. The present study analyzed rhizospheric soil of two kinds of plants bacterial diversity and community structure and their relationship with soil environmental factors. The results indicate that the rhizospheric soil bacteria species of Robinia pseudoacacia and Juniperus sabina were not significantly different from that of bare tailings, but rhizospheric soil bacterial community compositions and abundance were significantly different from that of bare tailings. Canonical correlation analysis (CCA) showed that soil alkali-hydrolyzable nitrogen (AN), soil total nitrogen (TN), and soil organic matter (SOM) were the main environmental factors affecting bacterial community diversity. Spearman\'s correlation analysis showed that AN, TN, and SOM were significantly positively correlated with the relative abundance of Gemmatimonadetes and Nitrospirae, and were significantly negatively correlated with that of Firmicutes, Fusobacteria, and Bacteroidetes. FAPROTAX function prediction showed that the functional microbial communities of rhizospheric soil of the two plants were significantly different from those of bare tailings. Overall, the findings support an increase of microbial diversity, SOM, and nitrogen in rhizospheric soil of revegetated tailings compared to bare tailings. These results provide theoretical support for the development and application of phytoremediation in abandoned mines.
摘要：
铁尾矿基质缺乏营养，植物修复是提高尾矿养分的有效方法之一。植物修复对尾矿微生物的响应仍有待研究。本研究分析了两种植物根际土壤的细菌多样性和群落结构及其与土壤环境因子的关系。结果表明，刺槐和刺槐根际土壤细菌种类与裸尾矿没有显着差异。但根际土壤细菌群落组成和丰度与裸尾矿有显著差异。典型相关分析(CCA)表明，土壤碱解氮(AN)，土壤全氮(TN)，土壤有机质(SOM)是影响细菌群落多样性的主要环境因子。斯皮尔曼相关分析表明，TN,和SOM与Gemmatatimonadetes和Nitrosirae的相对丰度呈显着正相关，与Firmicutes呈显著负相关，镰刀菌,和拟杆菌。FAPROTAX功能预测表明，两种植物根际土壤的功能微生物群落与裸尾矿差异显著。总的来说,这些发现支持微生物多样性的增加，SOM,与裸露的尾矿相比，再植被尾矿的根际土壤中的氮。研究结果为废弃矿山植物修复技术的发展和应用提供了理论支持。
