Abstract：
Multiple roles of reactive oxygen species (ROS) and their consequences for health and disease are emerging throughout biological sciences. This development has led researchers unfamiliar with the complexities of ROS and their reactions to employ commercial kits and probes to measure ROS and oxidative damage inappropriately, treating ROS (a generic abbreviation) as if it were a discrete molecular entity. Unfortunately, the application and interpretation of these measurements are fraught with challenges and limitations. This can lead to misleading claims entering the literature and impeding progress, despite a well-established body of knowledge on how best to assess individual ROS, their reactions, role as signalling molecules and the oxidative damage that they can cause. In this consensus statement we illuminate problems that can arise with many commonly used approaches for measurement of ROS and oxidative damage, and propose guidelines for best practice. We hope that these strategies will be useful to those who find their research requiring assessment of ROS, oxidative damage and redox signalling in cells and in vivo.
摘要：
活性氧（ROS）的多种作用及其对健康和疾病的影响正在整个生物科学中出现。这一发展导致研究人员不熟悉ROS及其反应的复杂性，他们采用商业试剂盒和探针来不适当地测量ROS和氧化损伤，将ROS（通用缩写）视为离散的分子实体。不幸的是,这些测量的应用和解释充满了挑战和局限性。这可能导致误导性主张进入文献并阻碍进展，尽管关于如何最好地评估个体ROS的知识体系已经成熟，他们的反应,作为信号分子和它们可能引起的氧化损伤。在这个共识声明中，我们阐明了许多常用的ROS和氧化损伤测量方法可能出现的问题，并提出最佳实践指南。我们希望这些策略对那些发现他们的研究需要评估ROS的人有用，细胞和体内的氧化损伤和氧化还原信号传导。
