A total of 47 patients with T1D, 30 patients with type 2 diabetes (T2D) and 24 healthy controls were recruited in this study. Flow cytometry was used to measure the levels of different B cell subsets (including B cells, plasmablasts, and Bregs) in the peripheral blood. Radiobinding assays were performed to detect the antibody titres of T1D patients. In addition, the correlations between different B cell subsets and patient parameters were investigated.
Compared with healthy controls, differences in frequency of Tim-1+ Bregs were significantly decreased in patients with T1D (36.53 ± 6.51 vs. 42.25 ± 6.83, P=0.02*), and frequency of IL-10+ Bregs were lower than healthy controls (17.64 ± 7.21vs. 24.52 ± 11.69, P=0.009**), the frequency of total Bregs in PBMC was also decreased in patients with T1D (1.42 ± 0.53vs. 1.99 ± 0.93, P=0.002.**). We analyzed whether these alterations in B cells subsets were associated with clinical features. The frequencies of Tim-1+ Bregs and IL-10+ Bregs were negatively related to fasting blood glucose (FBG) (r=-0.25 and -0.22; P=0.01* and 0.03*, respectively). The frequencies of Tim-1+ Bregs and IL-10+ Bregs are positively correlated with fast C-peptide (FCP) (r=0.23 and 0.37; P=0.02* and 0.0001***, respectively). In addition, the frequency of IL-10+ Breg was also negatively related to glycosylated haemoglobin (HbA1c) (r=-0.20, P=0.04*). The frequencies of Tim-1+ Bregs, IL-10+ Bregs and Bregs in T2D patients were reduced, but no statistically significant difference was found between other groups. Interestingly, there was positive correlation between the frequencies of Tim-1+ Bregs and IL-10+ Bregs in T1D (r=0.37, P=0.01*). Of note, it is worth noting that our study did not observe any correlations between B cell subsets and autoantibody titres.
Our study showed altered Tim-1 and IL-10 expression in regulatory B cell in T1D patients. Tim-1, as suggested by the present study, is associated with islet function and blood glucose levels. These findings indicate that Tim-1+ Bregs and IL-10+ Bregs were involved in the pathogenesis of T1D.
共有47例T1D患者,本研究招募了30名2型糖尿病(T2D)患者和24名健康对照。流式细胞术用于测量不同B细胞亚群(包括B细胞,成浆细胞,和Bregs)在外周血中。进行放射结合测定以检测T1D患者的抗体滴度。此外,研究了不同B细胞亚群与患者参数之间的相关性.
与健康对照相比,T1D患者的Tim-1+Bregs频率差异显着降低(36.53±6.51vs.42.25±6.83,P=0.02*),IL-10+Bregs的频率低于健康对照组(17.64±7.21vs.24.52±11.69,P=0.009**),T1D患者PBMC中总Bregs的频率也降低(1.42±0.53vs.1.99±0.93,P=0.002。**).我们分析了B细胞亚群的这些改变是否与临床特征相关。Tim-1+Bregs和IL-10+Bregs的频率与空腹血糖(FBG)呈负相关(r=-0.25和-0.22;P=0.01*和0.03*,分别)。Tim-1+Bregs和IL-10+Bregs的频率与快速C肽(FCP)呈正相关(r=0.23和0.37;P=0.02*和0.0001***,分别)。此外,IL-10+Breg的频率也与糖化血红蛋白(HbA1c)呈负相关(r=-0.20,P=0.04*)。Tim-1+Bregs的频率,IL-10+Bregs和Bregs在T2D患者中降低,但其他组间差异无统计学意义。有趣的是,T1D中Tim-1+Bregs和IL-10+Bregs的频率呈正相关(r=0.37,P=0.01*)。值得注意的是,值得注意的是,我们的研究未观察到B细胞亚群与自身抗体滴度之间存在任何相关性.
我们的研究显示T1D患者调节性B细胞中Tim-1和IL-10表达的改变。根据本研究的建议,Tim-1,与胰岛功能和血糖水平有关。这些发现表明Tim-1+Bregs和IL-10+Bregs参与了T1D的发病机制。