PDF(Pubmed)
Abstract：
Epidemiologic studies have indicated that cruciferous vegetables can influence the cancer risk; therefore, we examined with a cross-sectional approach the correlation between the frequent consumption of the total cruciferous vegetables and the formation of bulky DNA damage, a biomarker of carcinogen exposure and cancer risk, in the Gen-Air study within the European Prospective Investigation into Cancer and Nutrition (EPIC) cohort. DNA damage measurements were performed in the peripheral blood of 696 of those apparently healthy without cancer controls, including 379 never-smokers and 317 former smokers from seven European countries by the 32P-postlabeling assay. In the Gen-Air controls, the median intake of cruciferous vegetables was 6.16 (IQR 1.16−13.66) g/day, ranging from 0.37 (IQR 0−6.00) g/day in Spain to 11.34 (IQR 6.02−16.07) g/day in the UK. Based on this information, participants were grouped into: (a) high consumers (>20 g/day), (b) medium consumers (3−20 g/day) and (c) low consumers (<3.0 g/day). Overall, low cruciferous vegetable intake was correlated with a greater frequency of bulky DNA lesions, including benzo(a)pyrene, lactone and quinone-adducts and bulky oxidative lesions, in the adjusted models. Conversely, a high versus low intake of cruciferous vegetables was associated with a reduction in DNA damage (up to a 23% change, p = 0.032); this was particularly evident in former smokers (up to a 40% change, p = 0.008). The Generalized Linear Regression models indicated an overall Mean Ratio between the high and the low consumers of 0.78 (95% confidence interval, 0.64−0.97). The current study suggests that a higher intake of cruciferous vegetables is associated with a lower level of bulky DNA adducts and supports the potential for cancer prevention strategies through dietary habit changes aimed at increasing the consumption of cruciferous vegetables.
摘要：
流行病学研究表明，十字花科蔬菜可以影响癌症风险；因此，我们用横断面的方法检查了频繁食用十字花科蔬菜和形成庞大的DNA损伤之间的相关性，致癌物暴露和癌症风险的生物标志物，在欧洲癌症和营养前瞻性调查（EPIC）队列中的Gen-Air研究中。在696名没有癌症对照的健康患者的外周血中进行了DNA损伤测量，通过32P标记后检测，包括来自七个欧洲国家的379名从不吸烟者和317名前吸烟者。在Gen-Air控制中,十字花科蔬菜的平均摄入量为6.16（IQR1.16-13.66）g/天，范围从0.37（IQR0-6.00）g/天在西班牙到11.34（IQR6.02-16.07）g/天在英国。根据这些信息,参与者被分组为：(A)高消费者(>20克/天)，(b)中等消费者(3-20克/天)和(c)低消费者(<3.0克/天)。总的来说,低十字花科蔬菜的摄入量与更大的大的DNA损伤频率相关，包括苯并（a）芘，内酯和醌加合物和庞大的氧化损伤，在调整后的模型中。相反，十字花科蔬菜的高摄入量与低摄入量与DNA损伤的减少有关（变化高达23％，p=0.032)；这在前吸烟者中尤为明显(变化高达40%，p=0.008)。广义线性回归模型表明，高消费者和低消费者之间的总体平均比率为0.78(95%置信区间，0.64-0.97）。目前的研究表明，更高的十字花科蔬菜的摄入量与更低水平的庞大的DNA加合物有关，并通过改变饮食习惯来支持癌症预防策略的潜力，以增加十字花科蔬菜的消费。
