Abstract:
The catalytic activity of histone methyltransferases is not restricted to histones but also includes noncanonical substrates. Increasing evidence shows that histone methyltransferases methylate themselves, and automethylation has emerged as a self-regulatory mechanism. Here, we introduce experimental procedures to identify automethylation sites of histone methyltransferases and to investigate the function of automethylation in a reconstituted biochemical system and in cellular contexts.
摘要:
组蛋白甲基转移酶的催化活性不仅限于组蛋白,还包括非规范底物。越来越多的证据表明,组蛋白甲基转移酶本身甲基化,自甲基化已经成为一种自我调节机制。这里,我们介绍了实验程序,以确定组蛋白甲基转移酶的自甲基化位点,并研究自甲基化在重建的生化系统和细胞环境中的功能。