{Reference Type}: Journal Article
{Title}: Techniques to Study Automethylation of Histone Methyltransferases and its Functional Impact.
{Author}: Popoca L;Lee CH;
{Journal}: Methods Mol Biol
{Volume}: 2529
{Issue}: 0
{Year}: 2022
暂无{DOI}: 10.1007/978-1-0716-2481-4_5
{Abstract}: The catalytic activity of histone methyltransferases is not restricted to histones but also includes noncanonical substrates. Increasing evidence shows that histone methyltransferases methylate themselves, and automethylation has emerged as a self-regulatory mechanism. Here, we introduce experimental procedures to identify automethylation sites of histone methyltransferases and to investigate the function of automethylation in a reconstituted biochemical system and in cellular contexts.