Abstract：
The structure of the O-specific polysaccharide (OPS) from Aeromonas encheleia strain A4 lipopolysaccharide was investigated. A. encheleia strain A4, classified into the new provisional serogroup PGO1 predominating among aeromonads in Polish aquaculture, was isolated from common carp tissues during an outbreak of MAI/MAS disease on a fish farm. The high-molecular-weight OPS fraction liberated from the lipopolysaccharide after mild acid hydrolysis followed by gel-permeation chromatography was studied with chemical methods, mass spectrometry, and one- and two-dimensional 1H and 13C NMR spectroscopy techniques. Inter-residue correlations were identified in 1H,13C-heteronuclear multiple-bond correlation (HMBC) and 1H,1H NOESY experiments. It was found that the O-specific polysaccharide of A. encheleia strain A4 consists of branched pentasaccharide repeating units with the following structure:→2)[α-d-Fucp3NRHb-(1→3)]-α-l-Rhap-(1→3)-β-l-Rhap-(1→4)-α-l-Rhap-(1→3)-β-d-QuipNAc-(1→.
摘要：
研究了来自附生气单胞菌菌株A4脂多糖的O特异性多糖（OPS）的结构。A.encheleia菌株A4，被归类为新的临时血清群PGO1，在波兰水产养殖中占主导地位，在养鱼场爆发MAI/MAS疾病期间，从鲤鱼组织中分离出来。用化学方法研究了温和酸水解后，凝胶渗透色谱法从脂多糖中释放的高分子量OPS级分，质谱,以及一维和二维1H和13CNMR光谱技术。残基间相关性在1H，13C-异核多键相关（HMBC）和1H，1H噪声实验。发现A.encheleia菌株A4的O特异性多糖由具有以下结构的支链五糖重复单元组成：→2）[α-d-Fucp3NRHb-（1→3）]-α-1-Rhap-（1→3）-β-1-Rhap-（1→4）-α-1-Rhap1-（1→3）-β-
