Abstract：
OBJECTIVE: To elucidate the mechanism underlying how Ureaplasma urealyticum (UU) affects sperm quality and identify a therapeutic target.
METHODS: In this prospective observational study, the differences in and relationships among semen volume, pH, viscosity, liquefaction time, sperm concentration, sperm motility [progressive motility (PR)], and seminal polymorphonuclear (PMN) elastase were analyzed in 198 normal semen samples (control group) and 198 UU-infected semen samples (observation group). The UU-infected samples were treated and the above parameters were compared between the two groups.
RESULTS: The semen volume, viscosity, liquefaction time, and seminal PMN elastase were significantly higher in the observation than control group, but the pH and PR were significantly lower. In the observation group, the pH and PR were significantly higher after than before treatment, whereas the semen volume, PMN elastase, viscosity, and liquefaction time were lower. UU was closely related to semen volume, pH, viscosity, liquefaction time, sperm motility (PR), and PMN elastase. PMN elastase had significant negative effects on semen pH and sperm motility (PR) but positive effects on viscosity and liquefaction time.
CONCLUSIONS: UU might induce PMN elastase to increase the liquefaction time and viscosity of semen, eventually decreasing PR. PMN elastase might be a therapeutic target of UU.
METHODS: In this prospective observational study, the differences in and relationships among semen volume, pH, viscosity, liquefaction time, sperm concentration, sperm motility [progressive motility (PR)], and seminal polymorphonuclear (PMN) elastase were analyzed in 198 normal semen samples (control group) and 198 UU-infected semen samples (observation group). The UU-infected samples were treated and the above parameters were compared between the two groups.
RESULTS: The semen volume, viscosity, liquefaction time, and seminal PMN elastase were significantly higher in the observation than control group, but the pH and PR were significantly lower. In the observation group, the pH and PR were significantly higher after than before treatment, whereas the semen volume, PMN elastase, viscosity, and liquefaction time were lower. UU was closely related to semen volume, pH, viscosity, liquefaction time, sperm motility (PR), and PMN elastase. PMN elastase had significant negative effects on semen pH and sperm motility (PR) but positive effects on viscosity and liquefaction time.
CONCLUSIONS: UU might induce PMN elastase to increase the liquefaction time and viscosity of semen, eventually decreasing PR. PMN elastase might be a therapeutic target of UU.
摘要：
目的：阐明解脲支原体（UU）影响精子质量的潜在机制并确定治疗靶点。
方法：在这项前瞻性观察研究中，精液体积的差异和关系，pH值,粘度,液化时间,精子浓度,精子运动性[进行性运动性（PR）]，分析198例正常精液样本（对照组）和198例UU感染精液样本（观察组）中的多形核（PMN）弹性蛋白酶。对UU感染的样品进行处理,并在两组之间比较上述参数。
结果：精液体积，粘度,液化时间,和精浆PMN弹性蛋白酶明显高于对照组,但pH和PR显著降低。在观察组中,治疗后pH和PR明显高于治疗前，而精液体积，PMN弹性蛋白酶,粘度,液化时间较低。UU与精液体积密切相关，pH值,粘度,液化时间,精子运动性（PR），和PMN弹性蛋白酶。PMN弹性蛋白酶对精液pH和精子活力（PR）有明显的负面影响，但对粘度和液化时间有积极影响。
结论：UU可能诱导PMN弹性蛋白酶增加精液的液化时间和粘度，最终降低PR。PMN弹性蛋白酶可能是UU的治疗靶点。
方法：在这项前瞻性观察研究中，精液体积的差异和关系，pH值,粘度,液化时间,精子浓度,精子运动性[进行性运动性（PR）]，分析198例正常精液样本（对照组）和198例UU感染精液样本（观察组）中的多形核（PMN）弹性蛋白酶。对UU感染的样品进行处理,并在两组之间比较上述参数。
结果：精液体积，粘度,液化时间,和精浆PMN弹性蛋白酶明显高于对照组,但pH和PR显著降低。在观察组中,治疗后pH和PR明显高于治疗前，而精液体积，PMN弹性蛋白酶,粘度,液化时间较低。UU与精液体积密切相关，pH值,粘度,液化时间,精子运动性（PR），和PMN弹性蛋白酶。PMN弹性蛋白酶对精液pH和精子活力（PR）有明显的负面影响，但对粘度和液化时间有积极影响。
结论：UU可能诱导PMN弹性蛋白酶增加精液的液化时间和粘度，最终降低PR。PMN弹性蛋白酶可能是UU的治疗靶点。
