PDF(Pubmed)
Abstract：
UNASSIGNED: Bone defects remain an unsolved clinical problem due to the lack of effective osteogenic induction protocols. Nanomaterials play an important role in bone defect repair by stimulating osteogenesis. However, constructing an effective bioactive nanomaterial remains a substantial challenge.
UNASSIGNED: In this study, mesoporous silica nanoparticles (MSNs) were prepared and used as nanocarriers for basic fibroblast growth factor (bFGF). The characteristics and biological properties of the synthetic bFGF@MSNs were tested. The osteogenic effects of the particles on the behavior of MC3T3-E1 cells were investigated in vitro. In addition, the differentially expressed genes during induction of osteogenesis were analyzed by transcriptomic sequencing. Radiological and histological observations were carried out to determine bone regeneration capability in a distal femur defect model.
UNASSIGNED: Achieving bFGF sustained release, bFGF@MSNs had uniform spherical morphology and good biocompatibility. In vitro osteogenesis induction experiments showed that bFGF@MSNs exhibited excellent osteogenesis performance, with upregulation of osteogenesis-related genes (RUNX2, OCN, Osterix, ALP). Transcriptomic sequencing revealed that the Wnt/β-catenin signalling pathway could be activated in regulation of biological processes. In vivo, bone defect repair experiments showed enhanced bone regeneration, as indicated by radiological and histological analysis, after the application of bFGF@MSNs.
UNASSIGNED: bFGF@MSNs can promote bone regeneration by activating the Wnt/β-catenin signalling pathway. These particles are expected to become a potential therapeutic bioactive material for clinical application in repairing bone defects in the future.
UNASSIGNED: In this study, mesoporous silica nanoparticles (MSNs) were prepared and used as nanocarriers for basic fibroblast growth factor (bFGF). The characteristics and biological properties of the synthetic bFGF@MSNs were tested. The osteogenic effects of the particles on the behavior of MC3T3-E1 cells were investigated in vitro. In addition, the differentially expressed genes during induction of osteogenesis were analyzed by transcriptomic sequencing. Radiological and histological observations were carried out to determine bone regeneration capability in a distal femur defect model.
UNASSIGNED: Achieving bFGF sustained release, bFGF@MSNs had uniform spherical morphology and good biocompatibility. In vitro osteogenesis induction experiments showed that bFGF@MSNs exhibited excellent osteogenesis performance, with upregulation of osteogenesis-related genes (RUNX2, OCN, Osterix, ALP). Transcriptomic sequencing revealed that the Wnt/β-catenin signalling pathway could be activated in regulation of biological processes. In vivo, bone defect repair experiments showed enhanced bone regeneration, as indicated by radiological and histological analysis, after the application of bFGF@MSNs.
UNASSIGNED: bFGF@MSNs can promote bone regeneration by activating the Wnt/β-catenin signalling pathway. These particles are expected to become a potential therapeutic bioactive material for clinical application in repairing bone defects in the future.
摘要：
由于缺乏有效的成骨诱导方案,骨缺损仍然是未解决的临床问题。纳米材料通过刺激成骨在骨缺损修复中起重要作用。然而,构建有效的生物活性纳米材料仍然是一个巨大的挑战。
■在这项研究中，制备了介孔二氧化硅纳米颗粒（MSN）并用作碱性成纤维细胞生长因子（bFGF）的纳米载体。测试了合成的bFGF@MSNs的特性和生物学特性。体外研究了颗粒对MC3T3-E1细胞行为的成骨作用。此外,通过转录组测序分析诱导成骨过程中差异表达的基因。进行放射学和组织学观察以确定股骨远端缺损模型中的骨再生能力。
■实现bFGF持续释放，bFGF@MSNs具有均匀的球形形貌和良好的生物相容性。体外成骨诱导实验表明，bFGF@MSNs表现出优异的成骨性能，与成骨相关基因上调(RUNX2，OCN，Osterix,ALP)。转录组测序表明，Wnt/β-catenin信号通路可以在调节生物过程中被激活。在体内,骨缺损修复实验显示骨再生增强，如放射学和组织学分析所示，应用bFGF@MSNs后。
■bFGF@MSNs可通过激活Wnt/β-catenin信号通路促进骨再生。这些颗粒有望成为未来临床应用于修复骨缺损的潜在治疗性生物活性材料。
■在这项研究中，制备了介孔二氧化硅纳米颗粒（MSN）并用作碱性成纤维细胞生长因子（bFGF）的纳米载体。测试了合成的bFGF@MSNs的特性和生物学特性。体外研究了颗粒对MC3T3-E1细胞行为的成骨作用。此外,通过转录组测序分析诱导成骨过程中差异表达的基因。进行放射学和组织学观察以确定股骨远端缺损模型中的骨再生能力。
■实现bFGF持续释放，bFGF@MSNs具有均匀的球形形貌和良好的生物相容性。体外成骨诱导实验表明，bFGF@MSNs表现出优异的成骨性能，与成骨相关基因上调(RUNX2，OCN，Osterix,ALP)。转录组测序表明，Wnt/β-catenin信号通路可以在调节生物过程中被激活。在体内,骨缺损修复实验显示骨再生增强，如放射学和组织学分析所示，应用bFGF@MSNs后。
■bFGF@MSNs可通过激活Wnt/β-catenin信号通路促进骨再生。这些颗粒有望成为未来临床应用于修复骨缺损的潜在治疗性生物活性材料。
