RESULTS: Transcriptome and proteome profiling was performed on the basis of RNA-Seq and isobaric tag for relative and absolute quantitation technology to explore the molecular mechanisms of the response of Cucurbita ficifolia Bouché to Fusarium oxysporum f. sp. cucumerium infection. Comparative analyses revealed that 1850 genes and 356 protein species were differentially regulated at 2d and 4d after FOC inoculation. However, correlation analysis revealed that only 11 and 39 genes were differentially regulated at both the transcriptome and proteome levels after FOC inoculation at 2d and 4d, respectively. After FOC inoculation, plant hormones signal transduction, transcription factors were stimulated, whereas wax biosynthesis and photosynthesis were suppressed. Increased synthesis of oxidative-redox proteins is involved in resistance to FOC.
CONCLUSIONS: This study is the first to reveal the response of C. ficifolia leaf to FOC infection at the transcriptome and proteome levels, and to show that FOC infection activates plant hormone signaling and transcription factors while suppressing wax biosynthesis and photosynthesis. The accumulation of oxidative-redox proteins also plays an important role in the resistance of C. ficifolia to FOC. Results provide new information regarding the processes of C. ficifolia leaf resistance to FOC and will contribute to the breeding of cucumber rootstock with FOC resistance.
结果:在RNA-Seq和同量异位标签的基础上进行转录组和蛋白质组分析,用于相对和绝对定量技术,以探索南瓜对尖孢镰刀菌fsp。黄瓜感染。比较分析表明,在FOC接种后第2d和第4d,1850个基因和356个蛋白质种类受到差异调节。然而,相关分析显示,在第2天和第4天接种FOC后,只有11个和39个基因在转录组和蛋白质组水平上都有差异调控,分别。FOC接种后,植物激素信号转导,转录因子被刺激,而蜡的生物合成和光合作用受到抑制。氧化-氧化还原蛋白合成的增加涉及对FOC的抗性。
结论:这项研究首次揭示了直叶直叶在转录组和蛋白质组水平上对FOC感染的反应,并表明FOC感染激活植物激素信号和转录因子,同时抑制蜡的生物合成和光合作用。氧化-氧化还原蛋白的积累也在紫叶菜对FOC的抗性中起重要作用。结果提供了有关紫花苜蓿叶片对FOC抗性过程的新信息,并将有助于具有FOC抗性的黄瓜砧木的育种。